Recombined bursicon protein for facilitating increase of caridina antimicrobial peptide and application thereof

An antibacterial peptide, rice shrimp technology, applied in the biological field, can solve the problems of shrimp and crab anorexia, muscle white turbidity, body surface ulcer, etc., and achieve the effect of reducing mortality and improving antibacterial ability.

Inactive Publication Date: 2019-03-01
TIANJIN NORMAL UNIVERSITY
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of shrimp and crab breeding, diseases often occur, including bacterial diseases and viral diseases, which cause an

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Recombined bursicon protein for facilitating increase of caridina antimicrobial peptide and application thereof
  • Recombined bursicon protein for facilitating increase of caridina antimicrobial peptide and application thereof
  • Recombined bursicon protein for facilitating increase of caridina antimicrobial peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0032] Zhejiang rice prawns are purchased from the Zhejiang aquatic product market. They are about 1.5-2.5cm in length and weigh about 50-100mg each. They are healthy and vigorous. The rice prawns are purchased and kept in the laboratory. Water aeration, the temperature is about 26-28°C. Shrimp food was fed twice a day, and fresh aeration water was changed every two days.

[0033] Short distance: time is 3s, 1000rpm.

[0034] Bursicon-α has the DNA sequence described in SEQUENCE LISTING NO.1.

[0035] The Bursa protein has the amino acid sequence described in SEQUENCE LISTING NO.2.

[0036] Bursicon-β has the DNA sequence described in SEQUENCE LISTING NO.3.

[0037] The Bursβ protein has the amino acid sequence described in SEQUENCE LISTING NO.4.

[0038] The technical solution of the present invention will be further described below in conjunction with specific embodiments.

[0039] Step 1, Trizol method to extract total RNA

[0040] Take 1.5ml enzyme-free EP tube and place it in liqui...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Relative molecular weightaaaaaaaaaa
Relative molecular weightaaaaaaaaaa
Relative molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention discloses a recombined bursicon protein for facilitating increase of a caridina antimicrobial peptide and application thereof. A Bursalpha protein has an amino acid sequence shown as SEQUENCE LISTING NO.2, and a Bursabeta protein has an amino acid sequence shown as SEQUENCE LISTING NO.4. Both the Bursalpha protein and the Bursbeta have the beneficial effect of improving the gene expression amount of the caridina antimicrobial peptide. By adopting the recombined bursicon protein, the antibacterial performance of aquaculture shrimps and crabs is improved, the death rate in aquaculture production is lowered remarkably, and the shrimps and crabs can grow healthily, so that the culturing yield and profit are improved greatly.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a recombinant bursicon protein for promoting the increase of antimicrobial peptides of rice shrimp and an application thereof. Background technique [0002] In the process of aquaculture, diseases caused by negligent prevention often bring huge economic losses to farmers. The scale of aquaculture continues to expand, creating new ways for farmers to increase their economic income. However, the problems existing in the traditional farming mode make shrimp and crab farming often encounter the risk of various diseases. In the process of shrimp and crab breeding, diseases often occur, including bacterial diseases and viral diseases, which cause anorexia, gill rot, body surface ulcers, muscle whiteness, etc., and bring great losses to the breeding production. [0003] Crustaceans are invertebrates that mainly rely on their innate immune system to defend against pathogenic mic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K14/435C07K1/34
CPCC07K14/43509
Inventor 李冉孙金生翁洁羊王鑫
Owner TIANJIN NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap