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Recombined bursicon protein for facilitating increase of caridina antimicrobial peptide and application thereof

An antibacterial peptide, rice shrimp technology, applied in the biological field, can solve the problems of shrimp and crab anorexia, muscle white turbidity, body surface ulcer, etc., and achieve the effect of reducing mortality and improving antibacterial ability.

Inactive Publication Date: 2019-03-01
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of shrimp and crab breeding, diseases often occur, including bacterial diseases and viral diseases, which cause anorexia, gill rot, body surface ulcers, and white muscles of shrimp and crabs, which bring great losses to the breeding production.

Method used

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  • Recombined bursicon protein for facilitating increase of caridina antimicrobial peptide and application thereof
  • Recombined bursicon protein for facilitating increase of caridina antimicrobial peptide and application thereof
  • Recombined bursicon protein for facilitating increase of caridina antimicrobial peptide and application thereof

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Embodiment Construction

[0032] Zhejiang Rice Shrimp is purchased at the Zhejiang Aquatic Products Market. Its body length is about 1.5-2.5cm, and its weight is about 50-100mg each. It is healthy and energetic. The rice shrimps are raised in the laboratory after they are purchased. The water is aerated and the temperature is about 26-28°C. Shrimp food was fed twice a day, and fresh aeration water was replaced every two days.

[0033] Short distance: the time is 3s, 1000rpm.

[0034] bursicon-α has the DNA sequence described in SEQUENCE LISTING NO.1.

[0035] Bursa protein has the amino acid sequence described in SEQUENCE LISTING NO.2.

[0036] bursicon-β has the DNA sequence described in SEQUENCE LISTING NO.3.

[0037] Bursβ protein has the amino acid sequence described in SEQUENCE LISTING NO.4.

[0038] The technical solutions of the present invention will be further described below in conjunction with specific embodiments.

[0039] Step 1, Trizol method to extract total RNA

[0040] Take a 1.5m...

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Abstract

The invention discloses a recombined bursicon protein for facilitating increase of a caridina antimicrobial peptide and application thereof. A Bursalpha protein has an amino acid sequence shown as SEQUENCE LISTING NO.2, and a Bursabeta protein has an amino acid sequence shown as SEQUENCE LISTING NO.4. Both the Bursalpha protein and the Bursbeta have the beneficial effect of improving the gene expression amount of the caridina antimicrobial peptide. By adopting the recombined bursicon protein, the antibacterial performance of aquaculture shrimps and crabs is improved, the death rate in aquaculture production is lowered remarkably, and the shrimps and crabs can grow healthily, so that the culturing yield and profit are improved greatly.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a recombinant bursicon protein for promoting the increase of antimicrobial peptides of rice shrimp and an application thereof. Background technique [0002] In the process of aquaculture, diseases caused by negligent prevention often bring huge economic losses to farmers. The scale of aquaculture continues to expand, creating new ways for farmers to increase their economic income. However, the problems existing in the traditional farming mode make shrimp and crab farming often encounter the risk of various diseases. In the process of shrimp and crab breeding, diseases often occur, including bacterial diseases and viral diseases, which cause anorexia, gill rot, body surface ulcers, muscle whiteness, etc., and bring great losses to the breeding production. [0003] Crustaceans are invertebrates that mainly rely on their innate immune system to defend against pathogenic mic...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/34
CPCC07K14/43509
Inventor 李冉孙金生翁洁羊王鑫
Owner TIANJIN NORMAL UNIVERSITY
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