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Lactobacillus gasseri and application thereof in preparing premature delivery preventing medicine

A technology of Lactobacillus gasseri and medicines, applied in the field of medical microorganisms, can solve problems such as reducing the premature birth rate and developing drug resistance, and achieve the effects of ensuring the number of viable bacteria, inhibiting Gardnerella, and reducing the risk of premature birth

Active Publication Date: 2019-03-01
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The administration of antibiotics to treat abnormal flora can also effectively reduce the rate of premature birth. Therefore, it is more recommended to use antibiotics in the treatment of reproductive tract infections in pregnant women. However, long-term use of antibiotics will lead to drug resistance. New antimicrobial peptides have become a hot research topic at present.

Method used

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  • Lactobacillus gasseri and application thereof in preparing premature delivery preventing medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] This implementation is used to illustrate the antagonistic effect of the bacterial strain of the present invention on Gardnerella vaginalis. The Gardnerella vaginalis used in this example is the strain purchased from ATCC with the product number 10231.

[0047] Cultivate Lactobacillus gasseri H87 with MRS liquid medium to the concentration of bacteria

[0048] 1×10 7 CFU / mL culture medium, named as culture medium A.

[0049] Gardnerella vaginalis was cultured with MRS liquid medium to a cell concentration of 1×10 7 CFU / mL culture medium, named as culture medium B.

[0050] The above-mentioned culture solution A and the above-mentioned culture solution B were mixed in equal volumes as an experimental group, and the above-mentioned culture solution B alone was used as a control group.

[0051] 1 mL of the culture liquid of the experimental group and the control group were respectively inoculated into 150 mL of the MRS liquid medium, and cultured at 37° C. for 24 hours...

Embodiment 2

[0058] This example is used to illustrate the antagonistic effect of the strain of the present invention on Escherichia coli. The Escherichia coli used in this example is the strain purchased from ATCC with the product number 35218.

[0059] Lactobacillus gasseri H87 was cultured with MRS liquid medium to a concentration of 1×10 7 CFU / mL culture medium, named as culture medium A.

[0060] Escherichia coli was cultured with MRS liquid medium to a concentration of 1×10 7 CFU / mL culture medium, named as culture medium B.

[0061] The above-mentioned culture solution A and the above-mentioned culture solution B were mixed in equal volumes as an experimental group, and the above-mentioned culture solution B alone was used as a control group.

[0062] 1 mL of the culture liquid of the experimental group and the control group were respectively inoculated into 150 mL of the MRS liquid medium, and cultured at 37° C. for 24 hours to obtain a co-cultivation culture liquid.

[0063] T...

Embodiment 3

[0069] This example is used to illustrate the antagonistic effect of the strains of the present invention on Staphylococcus aureus. The Staphylococcus aureus used in this example is the strain purchased from ATCC with the product number 35218.

[0070] Lactobacillus gasseri H87 was cultured with MRS liquid medium to a concentration of 1×10 7 CFU / mL culture medium, named as culture medium A.

[0071] Staphylococcus aureus was cultured with MRS liquid medium to a concentration of 1×10 7 CFU / mL culture medium, named as culture medium B.

[0072] The above-mentioned culture solution A and the above-mentioned culture solution B were mixed in equal volumes as an experimental group, and the above-mentioned culture solution B alone was used as a control group.

[0073] 1 mL of the culture liquid of the experimental group and the control group were respectively inoculated into 150 mL of the MRS liquid medium, and cultured at 37° C. for 24 hours to obtain a co-cultivation culture liq...

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Abstract

The invention discloses female vaginal dominant bacteria lactobacillus gasseri H87 and application thereof in preparing a premature delivery preventing medicine, and belongs to the technical field ofmedical microorganisms. The L.gasseri H87 is a novel bacterial genus of a lactobacillus gasseri genus separated from a human body, a repelling effect is avoided, specifically, antibacterial peptide gassericin of the L.gasseri H87 maintains balance of a vaginal flora, and has higher capability of adhering to vaginal epithelial cells, the risk of inflammation of a urogenital region of a patient is reduced, meanwhile, the risk of pregnancy complications of a pregnant woman is reduced, and the strain can be stored for a long term, so that production is facilitated. Lactobacillus gasseri deficiencycan cause a bad pregnancy outcome, such as premature delivery, for the pregnant woman, and a female vaginal microenvironment is reshaped, and therefore the strain can well meet the actual clinical application requirements, and a novel idea is provided for reducing the premature birth rate.

Description

technical field [0001] The present invention relates to a kind of Lactobacillus gasseri and its application in the preparation of medicines for preventing premature birth, especially a kind of Lactobacillus gasseri (Lactobacillus gasseri) H87, which plays a role in regulating vaginal microenvironment, inhibiting Gardnerella vaginalis (BV pathogenic At the same time, it has the effect of obviously inhibiting Candida and pathogenic bacteria, and has the potential of unique therapeutic advantages for clinical gynecological diseases (preterm birth), and belongs to the field of medical microbiology technology. Background technique [0002] In 1961, WHO initiated the definition of preterm birth as delivery between 28 weeks of gestation (196d) and 37 weeks of gestation (259d), and the newborn was premature. Preterm birth includes spontaneous premature birth and iatrogenic premature birth caused by mother or fetus (induced labor or cesarean section accounts for about 30%-35%), and s...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P15/00A61P15/02A61P15/06A61P31/04C12R1/225
CPCA61P15/00A61P15/02A61P15/06A61P31/04A61K2035/115C12R2001/225C12N1/205
Inventor 曾骥孟宋萍赵烨胡鹏陈帅黄宝琪
Owner NANJING UNIV OF TECH
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