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Method for determining dihydropyridine in feed

A technology for dihydropyridine and a determination method, which is applied in the field of feed, can solve the problems of limited usage, no method for determining dihydropyridine, etc., and achieves the effects of a reliable method, good linear correlation and repeatability, and meeting monitoring requirements.

Active Publication Date: 2019-03-01
青岛谱尼测试有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] Although dihydropyridine has multiple functions, its usage is limited. According to the 2017 edition of the "National Veterinary Drug Standards", the allowable dosage of cattle is 100g-150g per 1000kg of feed, and 150g for broiler chickens. The drug withdrawal period for broilers is 7 days, and the milk abandonment period is 7 days
In the 315 Gala in 2017, it was exposed that some feed companies added a large amount of growth-promoting drugs in order to maximize profits, including dihydropyridine, but there is no method for determining dihydropyridine in feed

Method used

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  • Method for determining dihydropyridine in feed
  • Method for determining dihydropyridine in feed
  • Method for determining dihydropyridine in feed

Examples

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Embodiment 1

[0052] The detection method of dihydropyridine in embodiment 1 duck compound feed

[0053] 1. Sample extraction: Accurately weigh 2.0g (accurate to 0.01g) of evenly crushed feed sample into a 50mL polypropylene plastic centrifuge tube, add 10mL of 40% ethanol solution, vortex on a vortex mixer for 1min, ultrasonically extract for 10min, 6000 Centrifuge at rpm for 5min, and collect the supernatant in another 50mL centrifuge tube. Another 10 mL of 40% ethanol solution was added to the residue, the above extraction steps were repeated, and the supernatants were combined for purification.

[0054] 2. Purification of solid phase extraction column: take 2mL of supernatant, dilute to 10mL with water to obtain a dilution, pass all the dilution through a Thermo PEP solid phase extraction column, rinse with water, blow dry, and elute with 3mL of acetonitrile. Dilute to 5 mL with water, pass through a 0.22 μm organic filter membrane, transfer to a sample injection vial, and form a test ...

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Abstract

The invention discloses a method for determining dihydropyridine in feed. The method comprises the following steps: weighing a uniformly crushed feed sample, adding an organic solution for extractiontwice, and combining supernatants; after purification by a solid phase extraction column, collecting an eluate and performing volume fixation to a certain volume, and performing determining on a liquid chromatography-mass spectrometry / mass spectrometer. The defect of lack of a detection method for dihydropyridine in feed at present is overcome, the method has the characteristics of being simple and convenient to operate, high in sensitivity, high in recovery rate, accurate in qualitative and quantitative determination and the like, and requirements of veterinary drug detection can be met.

Description

Technical field: [0001] The invention belongs to the technical field of feed, and in particular relates to a method for measuring dihydropyridine in feed through a high performance liquid chromatography tandem mass spectrometer. Background technique: [0002] Dihydropyridine, light yellow powder or needle crystal, non-toxic, odorless, discolored when exposed to light, easily oxidized, soluble in hot ethanol, slightly soluble in water. It is the first veterinary drug growth-promoting additive approved by the Ministry of Agriculture for use in my country, and it was included in the 2003 edition of the Ministry of Agriculture's "Veterinary Drug Quality Standards". [0003] It is reported that dihydropyridine is a new type of green multifunctional feed additive, which has a wide range of biological functions such as promoting animal growth, improving fur quality, increasing lean meat percentage, improving reproductive performance, and disease prevention and resistance. At the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 宋薇阎玉林任宝国杨秀丽
Owner 青岛谱尼测试有限公司
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