Thin layer identification method for amino acid components in radix pseudostellariae

An amino acid and thin-layer identification technology, which is applied in the direction of material separation, material analysis, and measuring devices, can solve the problems of being unable to participate in the identification of similar families and genera, and achieve the effect of strong identification ability

Active Publication Date: 2019-03-01
福建省中医药科学院
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is a thin-layer identification method of amino acid components in Radix Pseudostellariae, which overcomes the defect that

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Thin layer identification method for amino acid components in radix pseudostellariae
  • Thin layer identification method for amino acid components in radix pseudostellariae
  • Thin layer identification method for amino acid components in radix pseudostellariae

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0035] Example 1: Thin-layer identification method for amino acid components in Radix Pseudostellariae

[0036] 1. Drying herbs

[0037] In summer, when most of the stems and leaves are withered, dig, wash, remove the fibrous roots, put them in boiling water and then dry them or directly dry them, and the water content should not exceed 12.0%.

[0038] 2. Preparation of test product

[0039] Take 1g of the test product powder, add 10ml methanol, warm soak, shake for 30 minutes, filter, and concentrate the filtrate to 1ml as the test product solution.

[0040] Take another lg of Pseudostellaria chinensis reference material, and prepare the reference material solution in the same way.

[0041] In addition, 1g each of the mixed counterfeit medicinal materials: Ophiopogon japonicus, Stemone, Pale Bamboo Leaf, and Silver Bupleurum, was made into a mixed counterfeit medicinal solution by the same method.

[0042] Weigh 12.5 mg each of arginine, alanine, valine, γ-aminobutyric acid and leucine a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a thin layer identification method for amino acid components in radix pseudostellariae. A mixed solution of n-butanol: chloroform: water: glacial acetic acid of 6:2.5:2.3:3 isused as a developing agent. Under the optimized thin layer chromatographic analysis conditions, various amino acids in the radix pseudostellariae are more effectively separated, and can be well distinguished from counterfeit medicinal materials such as ophiopogon japonicus, radix stemonae and lophatherum gracile and from similar medicinal materials like radices stellariae dichotomae.

Description

technical field [0001] The invention belongs to the technical field of identification of traditional Chinese medicinal materials, and in particular relates to a method for thin-layer identification of amino acid components in the medicinal material of Radix Pseudostellariae. Background technique [0002] Pseudostellariae Pseudostellariae Pseudostellaria heterophylla (Miq.) Dried root tuber of Pax ex Paxet Hoffm. For my country's traditional Chinese medicine [National Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China. 2015 edition, one part. Beijing: China Medical Science and Technology Press, 2015: 68. ]. Sweet, slightly bitter, flat. Return spleen, lung meridian. It has the effects of replenishing qi and invigorating the spleen, promoting body fluid and moistening the lungs. For deficiency of the spleen and fatigue, loss of appetite, weakness after illness, deficiency of Qi and Yin, spontaneous sweating and thirst, dryness of lung and dry cou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/90G01N30/94
CPCG01N30/90G01N30/94
Inventor 阚永军胡娟蒋畅庞文生杨晗
Owner 福建省中医药科学院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap