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Thin layer identification method for amino acid components in radix pseudostellariae

An amino acid and thin-layer identification technology, which is applied in the direction of material separation, material analysis, and measuring devices, can solve the problems of being unable to participate in the identification of similar families and genera, and achieve the effect of strong identification ability

Active Publication Date: 2019-03-01
福建省中医药科学院
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Problems solved by technology

[0004] The object of the present invention is a thin-layer identification method of amino acid components in Radix Pseudostellariae, which overcomes the defect that the existing TLC identification method cannot distinguish Radix Pseudostellaria from similar families and genera such as Yin Bupleurum and other medicinal materials

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  • Thin layer identification method for amino acid components in radix pseudostellariae
  • Thin layer identification method for amino acid components in radix pseudostellariae
  • Thin layer identification method for amino acid components in radix pseudostellariae

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Embodiment 1

[0035] Embodiment 1: TLC identification method of amino acid components in Radix Pseudostellariae Pseudostellariae of the present invention

[0036] 1. Herb drying

[0037] Excavate when most of the stems and leaves are withered in summer, wash them, remove fibrous roots, put them in boiling water for a while, and then dry them in the sun or directly in the sun. The water content should not exceed 12.0%.

[0038] 2. Preparation of test article

[0039] Take 1 g of the test product powder, add 10 ml of methanol, soak in warm, shake for 30 minutes, filter, and concentrate the filtrate to 1 ml as the test solution.

[0040] Another 1g of Pseudostellaria reference medicinal material was taken, and the reference medicinal material solution was prepared in the same way.

[0041] In addition, take 1 g each of the counterfeit medicinal materials Ophiopogon japonicus, Baibu, Danzhuye, and Yin Bupleurum, and make a mixed counterfeit medicinal material solution by the same method.

[...

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Abstract

The invention discloses a thin layer identification method for amino acid components in radix pseudostellariae. A mixed solution of n-butanol: chloroform: water: glacial acetic acid of 6:2.5:2.3:3 isused as a developing agent. Under the optimized thin layer chromatographic analysis conditions, various amino acids in the radix pseudostellariae are more effectively separated, and can be well distinguished from counterfeit medicinal materials such as ophiopogon japonicus, radix stemonae and lophatherum gracile and from similar medicinal materials like radices stellariae dichotomae.

Description

technical field [0001] The invention belongs to the technical field of identification of traditional Chinese medicinal materials, and in particular relates to a method for thin-layer identification of amino acid components in the medicinal material of Radix Pseudostellariae. Background technique [0002] Pseudostellariae Pseudostellariae Pseudostellaria heterophylla (Miq.) Dried root tuber of Pax ex Paxet Hoffm. For my country's traditional Chinese medicine [National Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China. 2015 edition, one part. Beijing: China Medical Science and Technology Press, 2015: 68. ]. Sweet, slightly bitter, flat. Return spleen, lung meridian. It has the effects of replenishing qi and invigorating the spleen, promoting body fluid and moistening the lungs. For deficiency of the spleen and fatigue, loss of appetite, weakness after illness, deficiency of Qi and Yin, spontaneous sweating and thirst, dryness of lung and dry cou...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/94
CPCG01N30/90G01N30/94
Inventor 阚永军胡娟蒋畅庞文生杨晗
Owner 福建省中医药科学院
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