Method for screening T1 generation tobacco strain of mixed library gene editing

Abstract

The invention discloses a method for screening a T1 generation tobacco strain of mixed library gene editing. The method comprises the steps of (1) collecting sample information by using a handheld computer and wirelessly connecting with a server terminal; (2) according to a wireless connection result between the handheld computer and the server terminal, managing a tobacco leaf sample to achieve screening, collection of the tobacco leaf sample, receipt, processing, storage, sample delivery, testing, storage of residual samples and disposal of the residual samples of the tobacco strain which requires tobacco leaf sample collection in rear time; feeding testing results back to tobacco planters, and completing the screening of the T1 generation tobacco strain of the mixed library gene editing. The method can achieve efficient and accurate screening of similar massive amount of the T1 generation tobacco strain of the mixed library gene editing.

Description

technical field [0001] The invention relates to the field of computer software, in particular to a method for screening a large number of tobacco strains for gene editing T1 generation of efficient and accurate mixed library in gene editing breeding. Background technique [0002] Gene editing breeding technology has been widely used in the genome modification of various plants, such as Arabidopsis, rice and tobacco. With the establishment of tobacco gene editing and breeding factories and the rapid advancement of material creation, a large number of gene editing materials have been created. These editing materials have become the focus of attention in the screening of tobacco plant phenotypes. There are differences in biochemical indicators. Therefore, how to efficiently and accurately screen tobacco strains from massive editing materials has become an urgent problem to be solved. [0003] Since the screening of tobacco strains involves multiple steps such as tobacco strai...

AI Technical Summary

Problems solved by technology

[0003] Since the screening of tobacco strains involves multiple steps such as tobacco strain selection, tobacco leaf sample collection, processing and analysis feedback, including tobacco strain selection that requires tobacco leaf sample collection, tobacco strain sampling marking, special phenotype recording, tobacco leaf collection and bagging, and tobacco leaf freezing , freeze-drying of tobacco leaves, grinding of tobacco leaves, storage of tobacco leaf powder, testing and processing of residual samples, and finally re-screening of massive tobacco strains according to the feedback of the test results of tobacco leaves. Traditionally, manual use of a marker pen is used to copy the code of the tobacco plant to the sample bag or sample tube, and then gradually enter the code into the computer terminal. These operations require high personnel quality and are prone to various errors, which seriously affect the sample management process. Efficient and accurate screening of editing materials affecting tobacco leaves

At the same time, there is a large randomness in the sampling and pre-processing process. If there is no unified management, it will bring instability to the subsequent detection.

These have seriously affected the establishment of tobacco gene editing breeding factories and the rapid advancement of material creation