Cystatin C latex-enhanced turbidimetry detection kit and preparation and application methods thereof

A detection kit and latex-enhanced technology, applied in the fields of genetic engineering technology and immunological assay analysis, can solve the problems of low sensitivity and low specificity of monoclonal antibody detection kits, and achieve improved application value, high sensitivity, good specific effect

Active Publication Date: 2019-03-01
ANHUI DAQIAN BIO ENG LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Accordingly, in view of the problems of low specificity of polyclonal antibody detection kits and low sensitivity of monoclonal antibody detection kits in the prior art, there is an urgent need for a detection ki

Method used

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  • Cystatin C latex-enhanced turbidimetry detection kit and preparation and application methods thereof
  • Cystatin C latex-enhanced turbidimetry detection kit and preparation and application methods thereof
  • Cystatin C latex-enhanced turbidimetry detection kit and preparation and application methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] A cystatin C latex-enhanced turbidimetric detection kit of this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0063] Reagent R1:

[0064] Tris-HCL buffer 10mM / L

[0065] PEG6000 5g / L

[0066] Sodium Thimerosal 0.1g / L

[0067] The solvent is purified water;

[0068] Reagent R2:

[0069]

[0070] Its solvent is purified water.

Embodiment 2

[0072] A cystatin C latex-enhanced turbidimetric detection kit of this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0073] Reagent R1:

[0074] PBS buffer 100mM / L

[0075] PEG8000 50g / L

[0076] Sodium azide 1g / L

[0077] The solvent is purified water;

[0078] Reagent R2:

[0079]

[0080] Its solvent is purified water.

Embodiment 3

[0082] A cystatin C latex-enhanced turbidimetric detection kit of this embodiment includes reagent R1 and reagent R2 two-liquid components that are independent of each other, and the components and corresponding contents included are:

[0083] Reagent R1:

[0084] PB buffer 50mM / L

[0085] DTT 25g / L

[0086] Gentamicin Sulfate 0.5g / L

[0087] The solvent is purified water;

[0088] Reagent R2:

[0089]

[0090] Its solvent is purified water.

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Abstract

The invention provides a cystatin C latex-enhanced turbidimetry detection kit. The cystatin C latex-enhanced turbidimetry detection kit comprises double liquid components, namely, a reagent R1and a reagent R2, wherein the reagent R1 is prepared from 10 to 100 mM/L buffer solution, 5 to 50g/L stabilizer and 0.1 to 1g/L preservative, wherein purified water is taken as the solvent; the reagent R2 isprepared from 10 to 100mM/L buffer solution, 0.1 to 1 percent surfactant, 5 to 50g/L stabilizer, 0.1 to 1g/L preservative, 1 to 10 percent latex microspheres crosslinking with a recombinant antihumanCys-C single-chain antibody, and 2 to 15 percent of latex microspheres crosslinking with a goat antihuman Cys-C polyclonal antibody, wherein purified water is taken as the solvent. The invention further provides preparation and application methods of the cystatin C latex-enhanced turbidimetry detection kit. By adopting the detection kit, the advantages of a polyclonal antibody detection kit and amonoclonal antibody detection kit are combined, and the specificity and sensitivity of detection can be enhanced simultaneously.

Description

technical field [0001] The invention relates to the fields of genetic engineering technology and immunological determination and analysis, in particular to a cystatin C latex enhanced turbidimetric detection kit and a preparation method thereof. Background technique [0002] Cystatin C (Cystatin C, Cys-C) is one of the members of cysteine ​​protease inhibitor superfamily 2 and is a cysteine ​​protease inhibitor composed of 122 amino acid residues. Products of housekeeping genes that are constantly expressed on nucleated cells. Cys-C in blood is basically filtered by glomerulus, therefore, the content of Cys-C in serum or plasma is completely determined by glomerular filtration rate (Glomeruar filtration rate, GFR). Because it has: ①stable production rate; ②stable blood concentration, not affected by other pathological changes, not combined with protein; ③glomerular free filtration, not reabsorbed or secreted; it is ideal for GFR detection Endogenous markers. Many recent s...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/531C12N15/70C12N15/13
CPCG01N33/531G01N33/54313G01N33/54393C12N15/70C07K16/38G01N2800/347Y02A50/30
Inventor 芮双印符修乐
Owner ANHUI DAQIAN BIO ENG LIMITED
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