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A kind of rpe cell sheet and its application and preparation method

A cell sheet and cell technology, applied in the medical field, can solve the problems of limited application and limited natural material donors, and achieve the effects of low immune rejection, good biocompatibility, and good biocompatibility

Active Publication Date: 2021-08-17
AIER EYE HOSPITAL GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited supply of natural materials, its application is limited.

Method used

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  • A kind of rpe cell sheet and its application and preparation method
  • A kind of rpe cell sheet and its application and preparation method
  • A kind of rpe cell sheet and its application and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0076] The preparation method of the present embodiment RPE cell sheet is as follows:

[0077] 1. After femtosecond surgery, corneal lens material itself and decellularized as scaffold material are as follows:

[0078] 1.1 After the full femtosecond laser operation, the thin layer of corneal tissue in the shape of a convex lens cut from the cornea was taken out and stored in normal saline or glycerin.

[0079] 1.2 Rinse with PBS 3 times, put in 1.5M NaCl solution, change it every 24 hours, shake for 48 hours in total.

[0080] 1.3 Shake with 5U / ml DNAse and 50μg / mL RNAse for 48 hours.

[0081] 1.4 Wash with PBS for 72 hours and change the solution every 24 hours.

[0082] 2. Using human umbilical vein endothelial cells (HUVEC) to construct a choroid-Bruch membrane composite structure:

[0083] 2.1 The lens is cut with a high-energy femtosecond laser or a blade, and a groove similar to a vascular network is obtained on the lens.

[0084]2.2 Inoculate HUVEC on the grooved le...

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Abstract

The invention relates to the field of medical technology, in particular to an RPE cell sheet and its application and preparation method. The RPE cell sheet comprises a corneal lens, a choroidal capillary layer and an RPE cell layer arranged in sequence; a vascular network groove is arranged on the surface of the corneal lens, and the choroidal capillary layer is filled in the groove. The RPE cell sheet of the present invention has good biocompatibility and low immune rejection, and the cell sheet is superior to cell sheets constructed in other ways in terms of barrier, phagocytosis and other functional detection. Vision problems of patients with retinal degenerative diseases such as macular degeneration; and corneal lens is abundant, so it is a good and cheap substitute for Bruch's membrane.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to an RPE cell sheet and its application and preparation method. Background technique [0002] The blood-retinal barrier (BRB) is divided into the inner blood-retinal barrier (iBRB) and the outer blood-retinal barrier (oBRB). The barrier function is an important basis for maintaining the physiological function of the retina and vision. The outer blood-retinal barrier is composed of mature retinal pigment epithelium (RPE), fenestrated choroidal endothelial cells (ECs), and Bruch's membrane, a highly organized basement membrane between two types of cells. The retinal pigment epithelium (RPE) is a single layer of pigment epithelial cells located between Bruch's membrane and photoreceptor cells. Its main function is to regulate the "visual cycle" and maintain normal visual physiological functions. Once the RPE cells are defective, the photoreceptor cells cannot metabolize normally, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/38A61L27/50C12N5/079
CPCA61L27/3604A61L27/3808A61L27/3813A61L27/50A61L2430/16C12N5/0621
Inventor 顾佳宁陈建苏唐仕波王译妮
Owner AIER EYE HOSPITAL GRP CO LTD
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