High-linolenic acid rape gene donor and application thereof

A gene donor, linolenic acid technology, applied in application, plant genetic improvement, angiosperm/flowering plants, etc., to achieve the application value, protect cardiovascular and cerebrovascular health, and increase the effect of food quality

Active Publication Date: 2019-03-22
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, except for transgenic means, there is no pu

Method used

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  • High-linolenic acid rape gene donor and application thereof
  • High-linolenic acid rape gene donor and application thereof
  • High-linolenic acid rape gene donor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1: Research and breeding process of R8Q10, YH2-Sap, YH2-5005

[0062] Through hybridization among rapeseed varieties, combined with a large number of FOSS 5500 near-infrared diffuse reflectance instruments, it was found in 2015 that three different sources, low erucic acid and low glucosinolate resources, had high linolenic acid content (Table 2). These materials were screened in 2016, 2017, and 2018. As a result, the frequency of single plants with high linolenic acid in their offspring became higher and higher, and gradually stabilized (Table 3). The overall quality of different strains detected by near-infrared is:

[0063] (1) YH2 / Qian 5005, abbreviated YH2-5005, linolenic acid content 14.5-16.0%, linoleic acid 18.5-19.7%, oleic acid 49.6-55.3%, oil content 43.9-46.5%;

[0064] (2) YH2 / Sapphire, referred to as YH2-sap, linolenic acid 13.0-14.9%, linoleic acid 17.0-21.3%, oleic acid 50.4%-62.4%, oil content 44.6-44.9%;

[0065] (3) R8Q10, pedigree Rfo / Qin 7...

Embodiment 2

[0078] Embodiment 2: the application of R8Q10 in breeding

[0079] By crossing R8Q10 with high-oil-content rape inbred line Shuang8, two lines with a linolenic acid content of about 15% were screened from the F3 generation of R8Q10-Shuang8. The test results are shown in Table 5. Its offspring also had lines with higher than 15% linolenic acid.

[0080] Table 5: The content of linolenic acid screened in the F3 generation of R8Q10-Double 8

[0081]

Embodiment 3

[0082] Embodiment 3: Breeding of high linolenic acid hybrids

[0083] Using YH2-5005, YH2-Sap to cross with Polima CMS a sterile line ZH9A, F 1 On behalf of male sterility, YH2-5005 and YH2-Sap were continued to be backcrossed for more than 2 generations, and the sterile lines YH2-5005A and YH2-SapA were initially selected.

[0084] Since R8Q10 itself contains a restorer gene for cytoplasmic male sterility (CMS), it is a restorer line for Ogura CMS and PolimaCMS. In the future, the restorer line R8Q10 can be used to pollinate the sterile lines YH2-5005A and YH2-SapA respectively to produce high linolenic acid rapeseed hybrids.

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Abstract

The invention discloses a high-linolenic acid rape gene donor. The high-linolenic acid rapeseed gene donor is named cabbage type rape Brassica napus high-linolenic acid rape R8Q10 in China center fortype culture collection, and a preservation number is CCTCC NO:P201820. The high-linolenic acid rape gene donor is used for breeding novel rape varieties, from pedigrees obtained through selfing, hybridization, backcross and the like, a novel high-linolenic acid strain is separated out, and is used for a conventional variety, or pollination control systems of self-incompatibility, male sterility and the like are used for breeding a hybrid so that the high-linolenic acid rape gene varieties with the linolenic acid content in fatty acid reaching 15%-17% or above can be obtained.

Description

technical field [0001] The invention belongs to the technical field of improvement and utilization of edible quality of crops, and in particular relates to a high linolenic acid rapeseed gene donor and its application in variety breeding. Background technique [0002] 1. Fatty acid composition of rapeseed [0003] Rapeseed (mainly Brassica napus L) is the second largest oil crop in China and the world, and the main source of edible vegetable oil. The composition of fatty acids in rapeseed oil directly determines its edible quality and nutritional value. The main fatty acids in rapeseed are the following seven kinds (Edited by Liu Houli, Genetics and Breeding of Rapeseed, Shanghai Science and Technology Press, 1985), which are palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1Δ9), linoleic acid (C18:2, Δ9, 12), α-linolenic acid (C18:3 Δ9, 12, 15), eicosenoic acid (C20:1Δ9) and erucic acid (C22: 1Δ9). Palmitic acid and stearic acid are saturated fatty acids, whi...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H5/00A01H6/20
CPCA01H1/02A01H1/04A01H5/00
Inventor 于澄宇练景龙任莉锁弓琼董军刚黄镇徐爱遐
Owner NORTHWEST A & F UNIV
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