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Liquid bacterial fertilizer obtained by fermenting garlic straw by using enterobacter ludwigii and preparation method thereof

A technology of Enterobacter ludwigii and Enterobacter dewighi, which is applied in the field of liquid bacterial fertilizer and its preparation, can solve the problems of low-efficiency utilization of garlic straw, environmental pollution, etc., and achieve the problem of resource utilization, decontamination, The effect of relieving the load bearing pressure

Active Publication Date: 2019-03-22
ECOLOGY INST SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the low-efficiency utilization of garlic stalks and the pollution to the environment, the present invention provides a liquid fermented fermentation medium that uses garlic stalks as the main fermentation substrate and Enterobacter ludwig with antifungal activity and plant growth-promoting strains. Bacterial fertilizer and preparation method thereof

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 A liquid bacterial fertilizer using Enterobacter ludwig to ferment garlic stalks and its preparation method

[0027] The liquid bacterial fertilizer comprises the following raw materials in parts by weight:

[0028] 30 parts of fresh garlic stalks, 15 parts of potassium dihydrogen phosphate, 10 parts of soybean flour, 6 parts of corn flour, 1.5 parts of glucose, 0.6 parts of yeast powder, 50 parts of Enterobacter ludwig LB fermentation broth, 230 parts of water; The preparation method of Ludwig enterobacter LB fermented liquid, uses LB culture medium to cultivate Ludwig enterobacter Enterobacter ludwigii B424, when OD 600 When it was 0.7, the Enterobacter ludwig LB fermentation liquid was obtained;

[0029] Its preparation method is as follows:

[0030] (1) Use LB medium to culture Enterobacter ludwigii, OD 600 When it is 0.7, the culture is terminated, and the fermentation liquid of Enterobacter ludwigii LB is obtained;

[0031] (2) Grind fresh garlic s...

Embodiment 2

[0036] Example 2 A liquid bacterial fertilizer using Enterobacter ludwig to ferment garlic stalks and its preparation method

[0037] The liquid bacterial fertilizer comprises the following raw materials in parts by weight:

[0038] 35 parts of fresh garlic straw, 15 parts of potassium dihydrogen phosphate, 10 parts of soybean flour, 6 parts of corn flour, 1.5 parts of glucose, 0.6 parts of yeast powder, 50 parts of Enterobacter ludwig LB fermentation broth, 230 parts of water;

[0039] The preparation method of the Enterobacter ludwig LB fermentation broth, using LB culture medium to cultivate Enterobacter ludwig Enterobacter ludwigii B424, when OD 600 When it was 0.6, the Enterobacter ludwig LB fermentation liquid was obtained;

[0040] Its preparation method is as follows:

[0041] (1) Use LB medium to culture Enterobacter ludwigii, OD 600 When it is 0.6, the culture is terminated, and the fermentation liquid of Enterobacter ludwigii LB is obtained;

[0042] (2) Grind...

Embodiment 3

[0047] Example 3 A liquid bacterial fertilizer using Enterobacter ludwig to ferment garlic stalks and its preparation method

[0048] The liquid bacterial fertilizer comprises the following raw materials in parts by weight:

[0049] 40 parts of fresh garlic straw, 15 parts of potassium dihydrogen phosphate, 10 parts of soybean flour, 6 parts of corn flour, 1.5 parts of glucose, 0.6 parts of yeast powder, 50 parts of Enterobacter ludwig LB fermentation broth, 230 parts of water;

[0050] The preparation method of the Enterobacter ludwig LB fermentation broth, using LB culture medium to cultivate Enterobacter ludwig Enterobacter ludwigii B424, when OD 600 When it was 0.8, the Enterobacter ludwig LB fermentation broth was obtained;

[0051] Its preparation method is as follows:

[0052] (1) Use LB medium to culture Enterobacter ludwigii, OD 600 When it is 0.8, the culture is terminated, and the fermentation liquid of Enterobacter ludwigii LB is obtained;

[0053] (2) Grind ...

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Abstract

The invention relates to a liquid bacterial fertilizer obtained by fermenting garlic straws by using enterobacter ludwigii and a preparation method thereof. The liquid bacterial fertilizer is preparedfrom garlic straw, potassium dihydrogen phosphate, soybean meal, corn flour, glucose, yeast powder, water and enterobacter ludwigii (LB) fermentation liquid, wherein the enterobacter ludwigii is Enterobacter ludwigii B424; the preservation number of the enterobacter ludwigii is CGMCC No.14915; the preservation institution of the enterobacter ludwigii is China General Microbiological Culture Collection Center; the preservation address is No. 3, No. 1 Courtyard, West Beichen Road, Chaoyang District, Beijing, China; the preservation date is November 16, 2017. Through the produced disease-resistant multifunctional liquid bacterial fertilizer capable of accelerating growth of crops, the application of chemical fertilizers and pesticides in farmlands is reduced; the farmland ecological environment is improved; the liquid bacterial fertilizer has great economic, social and ecological benefits.

Description

technical field [0001] The invention relates to the technical field of straw organic fertilizer production, in particular to a liquid bacterial fertilizer for fermenting garlic straw by Enterobacter ludwig and a preparation method thereof. Background technique [0002] China is the main production area and the largest exporter of garlic in the world, with an annual output of more than 11 million tons of garlic, accounting for more than 66% of the world's total production. Shandong is the main production area of ​​garlic in my country. The world produces more than 3.7 million tons of garlic straw every year. If it is not handled in time, it will cause serious environmental pollution and other ecological problems. Garlic stalks are rich in nutrients, and non-recycling causes serious waste of resources. [0003] At present, researchers have returned garlic straw to the field. For example, the publication number CN103238445A provides a method for planting selenium-enriched garl...

Claims

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Application Information

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IPC IPC(8): C05F17/00C05G3/00C12N1/20C12R1/01
CPCC05B7/00C05F17/00C05G3/00C05G5/20C12N1/20C05F11/00C05F11/08Y02W30/40
Inventor 周方园张新建吴晓青赵晓燕周红姿范素素张广志谢雪迎王加宁
Owner ECOLOGY INST SHANDONG ACAD OF SCI
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