A halophilic halobacteria w-y12 and its application

A technology of W-Y12, salt field bacteria, applied in the directions of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of crude oil degradation experiments and other problems

Active Publication Date: 2022-04-05
北京润世能源技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The literature "Study on the Diversity of Microbial Community Diversity of Suaeda Pterosa Root System and Its Loading Performance to Oil-Breaking Bacteria" reported the strains belonging to Salinicola zeshunii after comparison, but no crude oil degradation experiment was carried out

Method used

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  • A halophilic halobacteria w-y12 and its application
  • A halophilic halobacteria w-y12 and its application
  • A halophilic halobacteria w-y12 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Isolation, identification and preservation of embodiment 1, W-Y12 bacterial strain

[0049] 1. Isolation of W-Y12 strain

[0050] In July 2017, oil phase oil was obtained from the oil well production fluid of Shengli Oilfield in Karamay, Xinjiang, from which the inventor isolated a strain and named it strain W-Y12.

[0051] 2. Identification of W-Y12 strain

[0052] 1. Morphological identification of strain W-Y12

[0053] Morphological characteristics of the W-Y12 strain: yellow round raised colonies are formed on the LB plate containing 5% (mass fraction) sodium chloride.

[0054] 2. Physiological and biochemical identification of strain W-Y12

[0055] Physiological and biochemical characteristics of the W-Y12 strain: it can grow under the concentration of 3-20% sodium chloride.

[0056] 3. Molecular identification of strain W-Y12

[0057]The W-Y12 bacterial strain of activation is inoculated in the liquid LB culture medium (solvent is water, solute and concentrat...

Embodiment 2

[0064] Embodiment 2, W-Y12 bacterial strain emulsifies heavy oil

[0065] The oil in this example was collected from Shengli Oilfield in Karamay, Xinjiang in July 2017. The oil viscosity at 30°C is 29100mPa.s, which is extra heavy oil.

[0066] 1. Inoculate the activated W-Y12 into the liquid LB medium containing 5% (mass fraction) sodium chloride, culture at 30°C and 150 rpm for 24 hours with shaking, and obtain the W-Y12 bacterial liquid (bacterial concentration ≥ 10 8 cfu / mL).

[0067] 2. Test group: Inoculate 5 parts by volume of bacterial liquid to 95 parts by volume of petroleum-inorganic salt medium (solvent is water, solute and its concentration are respectively 1g / L petroleum, 94g / L NaCl, 1g / L NH 4 h 2 PO 4 , 1g / L (NH 4 ) 2 SO 4 , 1g / L K 2 HPO 4 、3g / LKNO 3 , total mineralization degree 100000mg / L, pH 7-8), 30°C, 150rpm shaking culture for 15d.

[0068] Control group: 100 parts by volume of petroleum-inorganic salt medium was cultured with shaking at 30° C. a...

Embodiment 3

[0071] Embodiment 3, the application of W-Y12 bacterial strain in microbial oil recovery technology

[0072] The oil in this example was collected from Shengli Oilfield in Karamay, Xinjiang in July 2017. The oil viscosity at 30°C is 29100mPa.s, which is extra heavy oil.

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Abstract

The invention discloses a halophilic halobacterium W-Y12 and its application. The classification of the halophilic halobacterium W-Y12 of the present invention is named as the salinefield fungus Salinicola sp., and the strain has been preserved in the General Microorganism Center of the China Committee for the Collection of Microbial Cultures on November 13, 2018, and the preservation number is CGMCC No. .16742. It is proved by experiments that the halophilic halobacterium W-Y12 of the present invention can emulsify crude oil, and can not only be used for microbial oil recovery, improve oil recovery rate, but also be used for the control and repair of oil pollution, and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of microbial oil recovery, in particular to a halophilic halobacterium W-Y12 and its application. Background technique [0002] Oil pollution is widely distributed, involving various industries such as oil extraction, oil refining, chemical industry, and machinery, as well as various polluting environments such as oil spills. Petroleum hydrocarbon components are complex and diverse, and are highly hydrophobic, making them difficult to be contacted, degraded, and utilized by ordinary microorganisms. Microbial oil recovery technology is to inject nutrients or microorganisms into the formation, and use the growth and metabolism activities of microorganisms in the oil reservoir to improve crude oil production and recovery. [0003] Traditional petroleum functional strains are mostly isolated from water and soil. Existing studies have shown that microorganisms are widely distributed in petroleum oil phase in ad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K8/582A62D3/02C12R1/01A62D101/20
CPCA62D3/02C09K8/582A62D2101/20C12R2001/01C12N1/205
Inventor 池昌桥
Owner 北京润世能源技术有限公司
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