Method for isolated culture of mesenchymal stem cells from fetal membranes

A technology for separating and culturing MSCs, applied in the field of separating and culturing mesenchymal stem cells, it can solve the problems of cell quality impact, complicated separation process, long operation time, etc., and achieve the effect of simplified separation process, high purity and strong proliferation ability.
CN109504656AInactive Publication Date: 2019-03-22CHENGDU QINGKE BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHENGDU QINGKE BIOTECH
Publication Date
2019-03-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for isolated culture of mesenchymal stem cells from fetal membranes. The method comprises the following steps: tissue separation, cleaning, culture, passage and cryopreservation. The invention provides a method for separating the mesenchymal stem cells, which are derived from an amniotic membrane, chorion laeve and decidua parietalis, from the fetal membranes. The conventional material positions are changed, and the separation process is simplified. The mesenchymal stem cells are extracted with a tissue block wall adherence method, the extraction method is simple and fast, introduction of an exogenous enzyme is not needed, so that risks of an exogenous substance for affecting quality of a cell preparation are reduced, and the prepared cells have high activity and purity and better proliferation capacity.
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Description

Technical field

[0001] The invention belongs to the technical field of mesenchymal stem cells, and specifically relates to a method for separating and culturing mesenchymal stem cells from fetal membranes. Background technique

[0002] Fetal membranes are closely related to the development of the fetus. They constitute the amniotic cavity that surrounds and protects the fetus. The thickness of the fetal membranes after separation from the uterine wall is about 200-300μm. It is composed of amniotic membrane / intermediate sponge layer / smooth chorion / trophoblast / decidual wall Membrane composition; current studies have shown that human amniotic membrane, chorion, and parietal decidua are rich in mesenchymal stem cells. Mesenchymal stem cells are a kind of pluripotent stromal cells with strong self-renewal ability and many Differentiation potential, able to differentiate into osteoblasts, chondrocytes and adipocytes, has a wide range of clinical applications.

[0003] Existing mesenchym...

Claims

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