Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for isolated culture of mesenchymal stem cells from fetal membranes

A technology for separating and culturing MSCs, applied in the field of separating and culturing mesenchymal stem cells, it can solve the problems of cell quality impact, complicated separation process, long operation time, etc., and achieve the effect of simplified separation process, high purity and strong proliferation ability.

Inactive Publication Date: 2019-03-22
CHENGDU QINGKE BIOTECH
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing isolation process of mesenchymal stem cells often uses exogenous enzymes to digest tissue blocks, but the isolation process is cumbersome, the operation time is long, and the residue of exogenous enzymes will also affect the quality of cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for isolated culture of mesenchymal stem cells from fetal membranes
  • Method for isolated culture of mesenchymal stem cells from fetal membranes
  • Method for isolated culture of mesenchymal stem cells from fetal membranes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for separating and culturing mesenchymal stem cells derived from decidua from fetal membranes, including the following steps:

[0042] (1) Collect maternal blood before delivery for infectious disease detection, including syphilis, AIDS, hepatitis B, hepatitis C, etc., and select healthy full-term placenta;

[0043] (2) Cut the fetal membrane tissue at the edge of the placenta, cut it to an appropriate size, wash the fetal membrane tissue with tissue cleaning solution to wash away the blood, and then remove the residual blood vessel tissue on the decidua surface of the subfetal membrane wall, and proceed again Wash, use tissue tweezers to peel off the decidual tissue, place it in a petri dish to clean and remove the trophoblast and blood vessels;

[0044] (3) Transfer the separated decidua tissue to a 50mL centrifuge tube and cut to about 3mm 3 Add tissue cleaning solution to the tissue block, centrifuge at 700×g for 5 min;

[0045] (4) Add serum-free medium to the cle...

Embodiment 2

[0050] A method for separating and culturing mesenchymal stem cells derived from amniotic membrane from fetal membranes, including the following steps:

[0051] (1) Collect maternal blood before delivery for infectious disease detection, including syphilis, AIDS, hepatitis B, hepatitis C, etc., and select healthy full-term placenta;

[0052] (2) Cut the fetal membrane tissue at the edge of the placenta, cut it to an appropriate size, wash the fetal membrane tissue with tissue cleaning solution to wash away the blood, then peel off the amniotic membrane tissue with tissue forceps, and place it in a plate for cleaning ;

[0053] (3) Transfer the separated amniotic membrane tissue to a 50mL centrifuge tube and cut to about 3mm 3 Add tissue cleaning solution to the tissue block, centrifuge at 700×g for 5 min;

[0054] (4) Add serum-free medium to the cleaned amniotic membrane tissue for resuspension, and then inoculate it into a culture flask at a density of 6mg / cm 2 , The ratio of medium...

Embodiment 3

[0059] A method for separating and culturing smooth chorionic mesenchymal stem cells from fetal membranes, including the following steps:

[0060] (1) Collect maternal blood before delivery for infectious disease detection, including syphilis, AIDS, hepatitis B, hepatitis C, etc., and select healthy full-term placenta;

[0061] (2) Cut the fetal membrane tissue at the edge of the placenta, cut it to an appropriate size, wash the fetal membrane tissue with tissue cleaning solution, wash off the blood, and then use tissue forceps to peel off the upper layer of amniotic membrane tissue, and then peel off the smooth chorion Layer, place it in a petri dish for cleaning and remove residual blood vessels;

[0062] (3) Transfer the separated smooth chorionic tissue to a 50mL centrifuge tube and cut to about 3mm 3 Add tissue cleaning solution to the tissue block, centrifuge at 700×g for 5 min;

[0063] (4) Add serum-free medium to the washed smooth chorionic tissue for resuspension, and then i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for isolated culture of mesenchymal stem cells from fetal membranes. The method comprises the following steps: tissue separation, cleaning, culture, passage and cryopreservation. The invention provides a method for separating the mesenchymal stem cells, which are derived from an amniotic membrane, chorion laeve and decidua parietalis, from the fetal membranes. The conventional material positions are changed, and the separation process is simplified. The mesenchymal stem cells are extracted with a tissue block wall adherence method, the extraction method is simple and fast, introduction of an exogenous enzyme is not needed, so that risks of an exogenous substance for affecting quality of a cell preparation are reduced, and the prepared cells have high activity and purity and better proliferation capacity.

Description

Technical field [0001] The invention belongs to the technical field of mesenchymal stem cells, and specifically relates to a method for separating and culturing mesenchymal stem cells from fetal membranes. Background technique [0002] Fetal membranes are closely related to the development of the fetus. They constitute the amniotic cavity that surrounds and protects the fetus. The thickness of the fetal membranes after separation from the uterine wall is about 200-300μm. It is composed of amniotic membrane / intermediate sponge layer / smooth chorion / trophoblast / decidual wall Membrane composition; current studies have shown that human amniotic membrane, chorion, and parietal decidua are rich in mesenchymal stem cells. Mesenchymal stem cells are a kind of pluripotent stromal cells with strong self-renewal ability and many Differentiation potential, able to differentiate into osteoblasts, chondrocytes and adipocytes, has a wide range of clinical applications. [0003] Existing mesenchym...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2509/00
Inventor 李荣张小虎海泉陈静娴
Owner CHENGDU QINGKE BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com