Intelligent molecular blotting affinity membrane for chiral resolution and its preparation method

A technology of chiral resolution and molecular imprinting, which is applied in chemical instruments and methods, membrane technology, semi-permeable membrane separation, etc., can solve problems such as hindering the application of solid membranes and weakening resolution capabilities, and achieves industrial production and high processing efficiency. Quantity, the effect of simple separation process

Inactive Publication Date: 2006-06-21

李连超

0 Cites 11 Cited by

AI-Extracted Technical Summary

Problems solved by technology

Usually one isomer can be more selectively adsorbed on the chiral selector, while the other isomer is more free in the polymer membrane. There are several preparations as follows: (1) immobilize chiral selectors, such as cyclodextrin and its derivatives, on the porous base membrane by grafting or staining (see: Kreig HM, Breytenbach J C, Keizer K, Chiralresolution by β -cyclodextrin polymer-impregnated ceramic membranes, Journal of Membrane Science, 2000, 164, 177-185), bovine serum albumin (BSA), apoenzyme and DNA, etc.; (2) Mix and dissolve cyclodextrin and other chiral selectors Using the membrane-making solution to make a non-porous polymer membrane (see Long Deyuan, Huang Tianbao, β-cyclodextrin polymer membrane for separation of amino acid enantiomers, Chemical Journal of Chinese Universities, 1999, 20: 884-886.) : (3) U...

Abstract

The present invention is chiral resolving affinity membrane for resolving, concentrating and separating chiral molecules and its preparation process. The present invention prepares temperature sensitive intelligent chiral resolving affinity membrane with porous inorganic or organic membrane as base membrane, and through heating polymerization or UV initiated polymerization of acrylate, acrylic acid, acrylamine, diacrylamine, isopropyl acrylamine, initiator and template molecule solution inside the pores and on the surface of the base membrane, and subsequent washing off the template molecule via altering the temperature. The preparation process includes the steps of: base membrane treatment, compounding monomer solution, polymerization and eliminating template. The intelligent chiral resolving affinity membrane has simple separation process, high chiral resolving capacity, high treating amount and no selectivity.

Application Domain

Semi-permeable membranes

Technology Topic

Chiral resolutionSeparation process +10

Examples

Experimental program(2)

Example Embodiment

[0027] Example 1

[0028] The smart molecular imprinting chiral resolution affinity membrane in this example uses a polyvinylidene fluoride porous membrane as the base membrane, and the base membrane is formed by thermal polymerization to contain polyisopropylacrylamide chains and template molecular imprinting chiral resolution. The preparation method of the affinity membrane includes the following process steps:

[0029] 1 Treatment of base film

[0030] Wash the base film with an ethanol solution with a volume concentration of 60% and dry it to a constant weight. The drying temperature is 50°C. The dried base film is placed in a base film bottle, and the base film bottle is replaced with high-purity nitrogen. Repeat 3 times to form a high-purity nitrogen atmosphere in the base film bottle;

[0031] 2 Preparation and treatment of monomer solution

[0032] Monomer solution preparation: pass the refined methyl acrylate, acrylic acid, acrylamide, isopropyl acrylamide and other monomers and crosslinking agents such as bisacrylamide, as well as the initiator and template molecule solution into the base film container, and then Perform high-purity nitrogen replacement in the base film bottle, and repeat 3 times to form a high-purity nitrogen atmosphere in the base film bottle;

[0033] Various monomers of methyl acrylate, acrylic acid, acrylamide, isopropyl acrylamide, the addition ratio is generally 20%;

[0034] The crosslinking agent includes ethylene glycol diacrylate or propylene glycol diacrylate or butylene diacrylate or bisacrylamide or the addition ratio is generally 8%;

[0035] Template molecules refer to adsorbed molecules, and the addition ratio is generally 10%;

[0036]Initiators include thermal initiators or UV photoinitiators, and the addition ratio is generally 5%;

[0037] 3 Aggregation

[0038] Place the container containing the base film bottle and the monomer solution in a 70°C constant temperature water bath for polymerization reaction, the reaction time is 6 hours, and then let the air into the atmosphere to stop the reaction;

[0039] 4 Cleaning of grafted membrane

[0040] The molecularly imprinted membrane is immersed in deionized water and shaken in a constant temperature water bath at 10°C to clean it. After cleaning, it is vacuum dried at 50°C to a constant weight to obtain a smart molecularly imprinted chiral separation affinity membrane.

Example Embodiment

[0041] Example 2:

[0042] The smart molecular imprinting chiral resolution affinity membrane in this embodiment uses a polyvinylidene fluoride porous membrane as the base membrane, and polymerization is initiated on the pores of the base membrane or on the surface of the membrane to obtain the smart molecular imprinting chiral resolution affinity membrane. And membrane, its preparation process steps are as follows:

[0043] 1 Treatment of base film

[0044] Wash the base film with an ethanol solution with a volume concentration of 60% and dry it to a constant weight. The drying temperature is 50°C. The dried base film is placed in a base film bottle, and the base film bottle is replaced with high-purity nitrogen. Repeat 3 times to form a high-purity nitrogen atmosphere in the base film bottle;

[0045] 2 Preparation and treatment of monomer solution

[0046] Monomer solution preparation: pass the refined methyl acrylate, acrylic acid, acrylamide, isopropyl acrylamide and other monomers and crosslinking agents such as bisacrylamide, as well as the initiator and template molecule solution into the base film container, and then Perform high-purity nitrogen replacement in the base film bottle, and repeat 3 times to form a high-purity nitrogen atmosphere in the base film bottle;

[0047] Various monomers: methyl acrylate, acrylic acid, acrylamide, isopropyl acrylamide, the addition ratio is generally 20%;

[0048] The crosslinking agent includes ethylene glycol diacrylate or propylene glycol diacrylate or butanediol diacrylate or bisacrylamide or the addition ratio is generally 8%;

[0049] Template molecules refer to adsorbed molecules, and the addition ratio is generally 10%;

[0050] Initiators include thermal initiators or UV photoinitiators, and the addition ratio is generally 5%;

[0051] 3 Aggregation

[0052] Place the container containing the base film bottle and the monomer solution in the UV initiating device, the UV initiation time is 120 seconds, and the distance between the UV light source and the container is 40 cm, and then the atmosphere is vented to stop the reaction;

[0053] 4 Cleaning of grafted membrane

[0054] The molecularly imprinted membrane is immersed in deionized water and shaken in a constant temperature water bath at 10°C to clean it. After cleaning, it is vacuum dried at 50°C to a constant weight to obtain a smart molecularly imprinted chiral separation affinity membrane.

PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.

Classification and recommendation of technical efficacy words

Simple separation process
Increase throughput

Method for extracting p-tertbutyl benzaldehyde from oxidation reactant of p-tertbutyl methylbenzene through integration of liquid-liquid extraction crystallization and decompression sidetrack rectification

InactiveCN103483146ASimple separation processsave water

Owner:江苏沿江化工资源开发研究院有限公司

Silica gel supporter, supported catalyst and preparation method thereof

InactiveCN108794667ASimple separation processImprove separation efficiency

Owner:CHINA PETROLEUM & CHEM CORP +1

Intelligent molecular blotting affinity membrane for chiral resolution and its preparation method

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Examples

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

Similar technology patents

Method for recycling nylon/polyolefin composite membrane by utilizing ionic liquid

Method for preparing sec-butylamine from etherified C4

Method for sensitized preparation of cis-vitamin D or its derivs. using cross-linked polymer photosensitizer

Method for preparing low-carbon olefin from synthesis gas

Hericium erinaceus isolate and isolating method and application thereof

Classification and recommendation of technical efficacy words

Culture medium for separating enterococcus and streptococcus from milk sample and preparation method of culture medium

Bio-catalytic deracemization preparation method of non-natural L-amino acid

Producing method for small molecule companion with gene engineering Escherichia coli

Method for extracting p-tertbutyl benzaldehyde from oxidation reactant of p-tertbutyl methylbenzene through integration of liquid-liquid extraction crystallization and decompression sidetrack rectification

Silica gel supporter, supported catalyst and preparation method thereof

Barrier first method for single damascene trench applications

Method for sending channel quality information in a multi-carrier radio communication system, corresponding mobile terminal and base station

System and method for asynchronous update of indexes in a distributed database

Stratospheric-based communication system for mobile users having adaptive interference rejection

Capture and elution of bio-analytes via beads that are used to disrupt specimens