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Radix scutellariae identification method and application thereof

An identification method and technology of Scutellaria baicalensis are applied in the identification method of Scutellaria baicalensis and its application field, and can solve the problems that the Scutellaria baicalensis detection method cannot detect and control the quality as a whole.

Inactive Publication Date: 2019-03-22
YANGTZE RIVER PHARM GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides an identification method of Scutellaria baicalensis, through which a large number of samples can be analyzed and processed in a short time, and the origin, processing and appearance of Scutellaria baicalensis can be effectively discriminated and distinguished, and the method basis is provided for the quality control of Scutellaria baicalensis and the division of grade quality standards; The problem that the Scutellaria baicalensis detection method cannot detect and control its quality as a whole

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  • Radix scutellariae identification method and application thereof
  • Radix scutellariae identification method and application thereof
  • Radix scutellariae identification method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0062] Embodiment 1 The establishment method and methodological investigation of Scutellaria baicalensis fingerprint

[0063] 1. The establishment method of Scutellaria baicalensis fingerprint

[0064] The establishment method of Scutellaria baicalensis fingerprint collection, comprises the following steps:

[0065] (1) Preparation of the test solution: take 20 batches of Scutellaria baicalensis from different origins in the above table 1 and pass through No. 3 pharmacopoeia sieve after crushing, take 0.2 g of Scutellaria baicalensis powder, weigh it accurately, place it in a 10mL volumetric flask, add the volume fraction 80% methanol aqueous solution, set the volume to the scale, weigh, ultrasonically treat for 40 minutes, cool to room temperature, make up the lost weight with 80% methanol aqueous solution, shake well, centrifuge at 4000r / min for 10 minutes, take the supernatant solution, through a 0.45 μm microporous membrane, and take the subsequent filtrate as the test so...

Embodiment 2

[0100] Example 2 The detection and methodological investigation of the multi-wavelength quantitative components of the Scutellaria baicalensis fingerprint

[0101] 1. Detection of multi-wavelength quantitative components

[0102] The retention times of baicalin, wogonin, baicalein and wogonin were 34.587min, 43.612min, 52.882min and 66.679min respectively. Turn on the DAD detector for full-wavelength scanning, compare the peak areas of each drug efficacy index component under different wavelength conditions, and finally choose to detect the content of baicalin, wogonin, baicalein, and wogonin at 277 nm.

[0103] 2. Research on multi-wavelength quantitative component detection methodology

[0104] 2.1 Linear inspection

[0105] Precisely measure an appropriate amount of reference substance solution containing baicalin, wogonin, baicalein, and wogonin, and dilute it step by step to form a series of concentration reference substance solutions, and perform sample injection analy...

Embodiment 3

[0146] Example 3 Establishment of Scutellaria baicalensis chemical pattern recognition method

[0147] 1. Identification of Scutellaria baicalensis by hierarchical cluster analysis (HCA)

[0148] 1.1 Farthest neighbor element - cosine method

[0149] Use the data management function of Waters empower workstation to obtain relevant information such as peak area and retention time of each component of Scutellaria baicalensis. Import the obtained data matrix into SPSS 19.0, and use the most distant neighbor element-cosine method to perform hierarchical clustering analysis on different batches of Scutellaria baicalensis samples. The dendrogram is as follows Figure 4 shown. When the distance scale is about 22, the samples are divided into three categories: S2 is Kuqin, S1 and S3 are Tiaoqin. Among them, the S1 category was identified as uncut medicinal materials and thick slices, with a thickness greater than 3 mm; S2 was identified as thin slices of Scutellaria baicalensis, wi...

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Abstract

The invention discloses a radix scutellariae identification method. According to the identification method, radix scutellariae is used for preparing a solution of a test product, solutions in which abaicalin reference substance, a wogonoside reference substance, a baicalein reference substance and a wogonin reference substance are dissolved are used as reference substance solutions, and through high performance liquid chromatograph measurement, liquid chromatograms of the solution of the test product and the reference substance solutions are obtained respectively; the obtained liquid chromatograms are guided into a traditional Chinese medicine chromatograph fingerprint similarity evaluation system for analysis, through analysis, a radix scutellariae fingerprint is obtained, and hierarchical cluster analysis or principal component analysis or partial least square analysis is adopted for processing data of the radix scutellariae fingerprint, so that classification identification is conducted on the radix scutellariae or processed products of the radix scutellariae. According to the radix scutellariae identification method, on the basis of building the radix scutellariae fingerprint,the radix scutellariae index component content is measured, and by combining different chemical mode identification methods, the origin places, processing and appearance characters of the radix scutellariae can be effectively judged and distinguished.

Description

technical field [0001] The invention relates to the field of drug analysis, in particular to an identification method of Scutellaria baicalensis and its application. Background technique [0002] Scutellaria baicalensis, the name of traditional Chinese medicine, is the dried root of Scutellaria baicalensis Georgi. It is mainly produced in Hebei, Shanxi, Inner Mongolia, Henan, Shaanxi and other places in my country, and can be planted in most provinces in the north. It has a wide range of clinical applications, and is often used for dampness and temperature, jaundice, diarrhea, cough due to lung heat, carbuncle sores, and fetal restlessness. In ancient and modern times, Scutellaria baicalensis is divided into two specifications, Kuqin and Tiaoqin, and it is believed that the curative effects are different, and the functions and indications are different. Tiaoqin is conical, the surface is brownish-yellow or yellow, and the cross-section is dark yellow; the old root of Kuqin...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 陈钟任晓亮赵倩游慧邱峰
Owner YANGTZE RIVER PHARM GRP CO LTD
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