ACE (angiotensin-converting enzyme) inhibitory peptide from broccoli protein and enzyme digestion metabolism product, preparation method and application thereof

Abstract

The invention discloses an ACE (angiotensin-converting enzyme) inhibitory peptide from broccoli protein and an enzyme digestion metabolism product, a preparation method and application thereof. The ACE inhibitory peptide is characterized in that the sequence is shown in SEQ ID NO.1; the sequences of the enzyme digestion metabolism product are shown in SEQ ID NO.2 to 5. The preparation method comprises the following steps of (1) using fresh stems and leaves of the broccoli as the raw materials, pressing and heating, and centrifuging and drying the precipitate, so as to obtain a protein crude extract of the broccoli; (2) treating the polypeptide extract in the stems and leaves of the broccoli by an enzyme method, so as to obtain the polypeptide extract of the broccoli; (3) performing ultrafiltration and gel filtration on the polypeptide extract of the broccoli, and collecting the component with highest ACE inhibiting activity; (4) performing high performance liquid chromatography separation and purification on the polypeptide extract of the broccoli, and collecting the component with highest ACE inhibiting activity, so as to obtain the ACE inhibitory peptide. The ACE inhibitory peptide has the advantages that by simulating the digestion of gastrointestinal tracts, the peptide metabolism product is obtained, and can be used for preparing the foods or medicines for assisting to reduce blood pressure; the structure is simple, the product is safe, and the activity is strong.

Description

technical field [0001] The invention relates to the technical field of food and medicine, in particular to an ACE inhibitory peptide derived from broccoli protein, an enzymatic cleavage metabolite of the ACE inhibitory peptide, and a preparation method and application thereof. Background technique [0002] Broccoli is a vegetable crop with strong seasonality. The flower bulbs are harvested from November to March of the next year. The stems and leaves of the plants after the flower bulbs are harvested are piled up in the field as waste, which is seriously Pollution of the environment in the main broccoli producing areas. According to calculations, the yield of broccoli stems and leaves is generally 2.5-3 tons per mu. Only based on the 70,000-mu broccoli planting base in Shangpan Town, Linhai City, Zhejiang Province, the total amount can reach 175,000-210,000 tons. Excessive accumulation of broccoli stems and leaves seriously affects the virtuous cycle of the ecological envir...

AI Technical Summary

Problems solved by technology

At present, there are few studies at home and abroad about the effective polypeptide extracts extracted from broccoli stems and leaves for the prevention and treatment of hypertension

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