Rapid Commassie brilliant blue staining solution

Abstract

The invention discloses a rapid Commassie brilliant blue staining solution, containing Coomassie brilliant blue, water, alcohol, acid and xylo-oligosaccharide. According to the invention, methyl alcohol and acetic acid are not used, thus being more economical and environment-friendly, furthermore, compared with general Commassie brilliant blue staining, the staining sensitivity is improved; xylo-oligosaccharide is added to the staining solution, so that staining is rapid, a protein band can be seen only after 5 minutes of staining, the sensitivity is high, and mass spectrum compatibility is good; decoloring is not needed after staining, observation can be performed by rinsing simply with distilled water, the operation is simple and convenient, and the efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of protein gel electrophoresis, and in particular relates to a Coomassie brilliant blue staining solution capable of completing protein staining within 5-12 minutes. Background technique [0002] Gel electrophoresis is currently the most widely used protein separation technique in proteomics research. After electrophoresis, the protein band and content should be observed by staining technique. There are currently a variety of protein staining methods, which are mainly divided into the following four types: organic dye staining, negative staining, silver staining, and fluorescent dye staining. Among them, Coomassie Brilliant Blue staining is the most commonly used organic reagent staining method. This method was first applied to protein staining on cellulose acetate sheets by Fazekas in 1963, and then Meyer et al. used a solution of methanol, acetic acid, and water to prepare CBB R-250 staining solution for...

AI Technical Summary

Problems solved by technology

However, the disadvantages of this method are as follows: the sensitivity of the first staining is not high, and the visualization of low-abundance proteins is poor

After the second CBB staining, the gel has a darker color background, which takes a long time to decolorize, which affects the efficiency of the experiment

In addition to the problems of dyeing sensitivity and environmental protection, the common problem for those skilled in the art is the problem of dyeing and decolorization time. The dyeing time usually needs to be more than 30 minutes to achieve better experimental results. At present, there is a technical solution of adding soluble starch. , such as a Coomassie Brilliant Blue staining solution and a dyeing method disclosed in patent CN104004382B, although the dyeing time can be shortened, it can only be shortened to 20 minutes, which cannot meet the requirements of rapid experimentation.

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