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Method for detecting anti-oxidization capability of honey

A technology of anti-oxidation ability and honey, which is applied in the field of electrochemical detection, can solve the problems of unsuitable anti-oxidation ability of honey, etc., and achieve the effect of simple detection method, high detection accuracy and high sensitivity

Active Publication Date: 2019-03-26
山西圣康生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the applicant's previous patent application, the cross-linked hydrogel was used to detect the total antioxidant capacity of complex samples. Sodium phosphate, carrageenan, gelatin and carrageenan are not suitable for the detection of antioxidant capacity of honey

Method used

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  • Method for detecting anti-oxidization capability of honey
  • Method for detecting anti-oxidization capability of honey
  • Method for detecting anti-oxidization capability of honey

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Comparing the antioxidant capacity of two honeys from cotton and jujube nectar sources.

[0029] (1) Preparation of Guar Gum-Ag + Hydrogels:

[0030] Add 0.1mL AgNO to 6mL guar gum solution (1wt%) 3 (0.1mmol / L) solution, while stirring, add dropwise 0.1mmol / L dilute nitric acid solution to adjust the pH to 5.5 to obtain guar gum-Ag + Hydrogels.

[0031] (2) Measure the background signal:

[0032] The prepared 2mL guar gum-Ag + The hydrogel was used as an electrochemical detection platform, and 10 μL of 0.1 mmol / L Fenton’s solution was added for degradation reaction. After 3 minutes, a cyclic voltammetry scan was performed, and the obtained Ag / Ag+ redox signal was the background signal. Wherein, the scanning interval of the voltammetric scanning is 1.0V-0.0V; the scanning speed is 50mV / s.

[0033] (3) Measure the sample signal:

[0034] The prepared 2mL guar gum-Ag + The hydrogel is used as an electrochemical detection platform, add 10 μL 0.1mmol / L Fen...

Embodiment 2

[0038] Example 2: Comparing the antioxidant capacity of two kinds of honey from Robinia pseudoacacia and Vitex tea nectar sources.

[0039] (1) Preparation of Guar Gum-Ag + Hydrogels:

[0040] Add 0.1mL AgNO to 6mL guar gum solution (0.5wt%) 3 (0.05mmol / L) solution, while stirring, add dropwise 0.1mmol / L dilute nitric acid solution to adjust the pH to 5.5 to obtain guar gum-Ag + Hydrogels.

[0041] (2) Measure the background signal:

[0042] The prepared guar gum-Ag + The hydrogel was used as an electrochemical detection platform, and 15 μL of 0.1 mmol / L Fenton’s solution was added for degradation reaction. After 4 minutes, voltammetry and voltammetry scanning were performed to obtain Ag + The electrochemical signal during the depolymerization reaction is the background signal. Wherein, the scanning interval of the voltammetric scanning is 1.0V-0.0V; the scanning speed is 80mV / s.

[0043] (3) Measure the sample signal:

[0044] The prepared chitosan-Ag + The hydrogel ...

Embodiment 3

[0048] Example 3: Comparison of the antioxidant capacity of two types of honey from citrus and linden nectar sources.

[0049] (1) Preparation of Guar Gum-Ag + Hydrogels:

[0050] Add 0.1mL AgNO to 6mL guar gum solution (2wt%) 3 (0.15mmol / L) solution, while stirring, add dropwise dilute nitric acid solution to adjust the pH to 5.5 to obtain guar gum-Ag + Hydrogels.

[0051] (2) Measure the background signal:

[0052] The prepared guar gum-Ag + The hydrogel was used as an electrochemical detection platform, and 10 μL 0.15 mmol / L Fenton was added for depolymerization reaction, and cyclic voltammetry scanning was performed after 5 minutes to obtain Ag / Ag + The electrochemical signal during redox is the background signal. Wherein, the scanning interval of the voltammetric scanning is 1.0V-0.0V; the scanning speed is 20mV / s.

[0053] (3) Measure the sample signal:

[0054] The prepared guar gum-Ag + The hydrogel is used as an electrochemical detection platform. Add 10 μL 0...

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Abstract

The invention discloses a method for detecting an anti-oxidization capability of honey. The method comprises the following steps: taking guar gum-Ag<+> hydrogel as an electrochemical detection platform; adding a quantitative amount of Fenton and carrying out depolymerization reaction; meanwhile, measuring an Ag / Ag<+> oxidization reduction signal as a background signal; adding an equal amount of the Fenton solution and a quantitative amount of honey to be detected into the hydrogel, so as to obtain an Ag / Ag<+> oxidized and reduced electrochemical signal; dividing by the background signal to obtain the anti-oxidization performance of the honey, wherein the magnitude of an obtained value represents the intensity of the anti-oxidization capability of the honey, the total anti-oxidization capability of the honey is weaker when the value is smaller and the total anti-oxidization capability is stronger when the value is larger. The detection method provided by the invention is simple and feasible and the stability is higher than that of other common hydrogel; the anti-oxidization capability of the honey from different honey sources can be rapidly determined.

Description

technical field [0001] The invention belongs to the technical field of electrochemical detection, in particular to a method for detecting the antioxidant capacity of honey. Background technique [0002] Honey is a nutrient-rich natural food. Since ancient times, there have been records in my country that using honey as a dietary therapy can "invigorate Qi and nourish the middle, and take a long time to lighten the body and prolong life". There are very good curative effect. With the deepening of honey research, it is found that the reason why honey has certain effects on the above-mentioned diseases and prolonging life is not only because honey has antibacterial properties, but also because it has certain antioxidant activities. The antioxidant ability provides a theoretical basis for the health care function of honey. Due to the difference of bee species, nectar source and environment, the chemical composition of honey is very different, and the antioxidant ability is also ...

Claims

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Application Information

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IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 傅力吴梦瑶
Owner 山西圣康生物科技股份有限公司
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