A kind of plant stem cell active ingredient and preparation method thereof

A technology of plant stem cells and active ingredients, which is applied in the field of extraction and utilization of natural products, can solve problems such as impact on application, limitation of comprehensive efficacy of active ingredients, etc., and achieve the effect of strong function, excellent activity and anti-aging effect, and low cost.

Active Publication Date: 2022-05-20
东莞东芳漾生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, the growth of plants is affected by various factors such as environment and temperature. Due to the instability of their growth environment, there will be certain differences in the types and contents of active ingredients in plants, which will also affect their subsequent applications.
Moreover, the prior art usually uses organic solvents to extract one or several active ingredients in plants, which will also play a certain role in limiting the comprehensive efficacy of active ingredients.

Method used

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  • A kind of plant stem cell active ingredient and preparation method thereof
  • A kind of plant stem cell active ingredient and preparation method thereof
  • A kind of plant stem cell active ingredient and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] This embodiment relates to the preparation method of the plant stem cell active ingredient of the present invention, comprising the following steps:

[0035] 1) Inoculate apple stem cells in a liquid medium, and the composition of the medium is MS liquid medium+2,4-D 2.0mg / L+BA 1.0mg / L+CH1.0mg / L+AC 1.0mg / L, the pH value is 6.0, the cells are cultured at a temperature of 17.5°C, the light intensity is controlled at 1000Lux, the light is 16h, the dark is 8h, and the concentration of stem cells is 2×10 7 individual / ml, end the culture;

[0036] 2) Filter the stem cells to remove water, then place them at a temperature of -15°C, freeze them in about 25 minutes, maintain the frozen state for 2 hours, and then thaw them at 30°C, the resulting liquid is the The above-mentioned active ingredients of plant stem cells. Its electron microscope picture see figure 2 , the electron micrographs of stem cells are shown in figure 1 ,Depend on figure 1 and figure 2 It can be see...

Embodiment 2

[0038] This embodiment relates to the preparation method of the plant stem cell active ingredient of the present invention, comprising the following steps:

[0039] 1) Inoculate pea stem cells in liquid medium and culture them at a temperature of 17°C, control the light intensity to 2000 Lux, light for 16 hours, and dark for 8 hours, and cultivate until the concentration of stem cells is 2.5×10 6 individual / ml, end the culture;

[0040] 2) Filter the stem cells to remove water, then place them at -20°C, complete freezing in about 20 minutes, maintain the frozen state for 3 hours, and then thaw at 26°C, the obtained liquid is the The above-mentioned active ingredients of plant stem cells.

Embodiment 3

[0042] This embodiment relates to the preparation method of the plant stem cell active ingredient of the present invention, comprising the following steps:

[0043] 1) Strawberry stem cells were inoculated in liquid medium and cultured at a temperature of 17°C. The light intensity was controlled at 3000 Lux, the light was 16 hours, and the dark treatment was 8 hours. The concentration of stem cells was cultivated to 5×10 7 individual / ml, end the culture;

[0044] 2) The plant stem cells in a vigorous growth state are frozen at -10°C for about 30 minutes, maintained in a frozen state for 5 hours, and then thawed at 30°C, and the obtained liquid is the active ingredient of the plant stem cells .

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Abstract

The invention relates to a preparation method of active ingredients of plant stem cells. Plant stem cells in a vigorous growth state are frozen within 20 to 30 minutes under the condition of -20 to -10°C, maintained in the frozen state for 1 to 5 hours, and then frozen at 26 Thawing is carried out under the condition of ~30° C., and the obtained liquid is the active ingredient of the plant stem cells. The extract prepared by the method of the present invention can improve the metabolic mechanism of the skin, can adjust the skin without penetrating into the skin, has high safety, and exhibits significant effects in anti-aging and anti-hair loss, and has Wide application prospects. Moreover, the cultivation method of the present invention has high controllability and can ensure the batch stability of the product.

Description

technical field [0001] The invention relates to the technical field of extraction and utilization of natural products, in particular to a plant stem cell active ingredient and a preparation method thereof. Background technique [0002] Plant cells contain a variety of functional ingredients that are beneficial to the human body. Extracting active ingredients from plants has a long history of application, such as traditional Chinese medicine in my country. With the development of science and technology, extracting active ingredients from plants for application has made great progress no matter in terms of extraction methods or application fields. From the perspective of extraction methods, from traditional water extraction to organic solvent extraction, microwave extraction, ultrasonic extraction and supercritical extraction, its application has also expanded from traditional disease treatment to food, cosmetics, etc. field. [0003] However, there are various problems in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/99A61Q19/00A61Q7/00A61Q19/08
CPCA61Q7/00A61Q19/00A61Q19/005A61Q19/08A61K8/99A61K2800/84
Inventor 杨芳
Owner 东莞东芳漾生物科技有限公司
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