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Concentrated growth factor sustained-release freeze-dried film and preparation method thereof

A growth factor and slow-release technology, which is applied in the direction of pharmaceutical formulations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve problems such as limited application, poor mechanical properties, and rapid release of concentrated growth factors

Active Publication Date: 2020-11-10
STOMATOLOGY AFFILIATED STOMATOLOGY HOSPITAL OF GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, although the concentrated growth factors obtained by a specific centrifugation method can promote soft tissue healing, there are problems such as fast release of concentrated growth factors, poor mechanical properties, and inability to store for a long time, which seriously limit its clinical application.

Method used

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  • Concentrated growth factor sustained-release freeze-dried film and preparation method thereof
  • Concentrated growth factor sustained-release freeze-dried film and preparation method thereof
  • Concentrated growth factor sustained-release freeze-dried film and preparation method thereof

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preparation example Construction

[0030] The preparation method of the concentrated growth factor sustained-release freeze-dried film of one embodiment includes the following steps S110 to S140:

[0031] S110. Provide concentrated growth factors.

[0032] In this embodiment, the concentrated growth factor is prepared by the following method:

[0033] Provide blood samples;

[0034] Centrifuge the blood sample to obtain concentrated growth factors.

[0035] It should be noted that in this embodiment, a specific centrifugal device (Mcdifuge, Silfradentsrl, Italy) is used for centrifuging the blood sample, which can separate the concentrated growth factors in the blood sample well.

[0036] It is understandable that in other embodiments, other methods can also be used, as long as the concentrated growth factors in the blood sample can be separated.

[0037] S120: Disperse the concentrated growth factor in the aqueous solution of sodium alginate to obtain a mixed solution.

[0038] Wherein, the concentration of sodium alginate...

Embodiment 1

[0057] (1) Put 5ml venous blood sample into a special centrifugal device (Mcdifuge, Silfradentsrl, Italy) for centrifugal separation to obtain concentrated growth factors;

[0058] (2) Disperse the above-mentioned concentrated growth factor in an aqueous solution of sodium alginate (the concentration of sodium alginate in the aqueous solution of sodium alginate is 0.02 g / mL) to obtain a mixed solution;

[0059] (3) Stirring to CaCl at 550rpm 2 Aqueous solution (CaCl 2 Ca in aqueous solution 2+ The concentration of 0.5mol / L) is added dropwise to the above mixed solution, and the dropping acceleration rate is controlled to be 5.5mL / min, so that the sodium alginate is coated with the concentrated growth factor, and the concentrated growth factor and sodium alginate with a particle size of about 100μm are obtained. Of composite microspheres.

[0060] (4) Disperse the composite microspheres in an aqueous solution of chitosan (the concentration of chitosan in the aqueous solution of chitos...

Embodiment 2

[0068] (1) Put 5ml venous blood sample into a special centrifugal device (Mcdifuge, Silfradentsrl, Italy) for centrifugal separation to obtain concentrated growth factors;

[0069] (2) Disperse the above-mentioned concentrated growth factor in an aqueous solution of sodium alginate (the concentration of sodium alginate in the aqueous solution of sodium alginate is 0.01 g / mL) to obtain a mixed solution;

[0070] (3) Stirring to CaCl at a stirring rate of 1000rpm 2 Aqueous solution (CaCl 2 Ca in aqueous solution 2+ The concentration of 1mol / L) is added dropwise to the above mixed solution, and the dropping acceleration rate is controlled to 10mL / min, so that the sodium alginate is coated with the concentrated growth factor, and the composite of the concentrated growth factor and sodium alginate with a particle size of about 10μm Microspheres.

[0071] (4) Disperse the above-mentioned composite microspheres in an aqueous solution of chitosan (the concentration of chitosan in the aqueous...

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Abstract

A concentrate growth factor slow release freeze-dried membrane and a preparation method therefor. The preparation method comprises the following steps: providing concentrate growth factors; dispersing the concentrate growth factors in an aqueous solution of sodium alginate to obtain a mixed solution; dropwise adding the mixed solution to an aqueous solution containing divalent metal cations while stirring so as to enable the sodium alginate to clad the concentrate growth factors and obtain composite microspheres of the concentrate growth factors and the sodium alginate; and dispersing the composite microspheres in an aqueous solution of chitosan, and performing freeze drying so as to obtain the concentrate growth factor slow release freeze-dried membrane. The concentrate growth factor slow release freeze-dried membrane prepared by the method has the advantages that the release rate of the concentrate growth factors is controllable, the mechanical property is good, and the membrane can be stored for a long term.

Description

Technical field [0001] The invention relates to the technical field of biological cells, in particular to a concentrated growth factor slow-release freeze-dried film and a preparation method thereof. Background technique [0002] In 2006, Sacco produced concentrated growth factor CGF (Concentrate Growth Factors) by passing blood through a specific centrifugal device (Mcdifuge, Silfradentsrl, Italy), which has the following advantages: 1) It is taken from the patient's autologous venous blood, without immune rejection and ethical issues; 2) Do not add any foreign substances, no cross infection; 3) Contain high concentration of growth factors and CD34 stem cells, which can promote the healing of mucosa and bone tissue and improve the quality of bone formation; 4) The three-dimensional matrix network structure of fibrous protein is very dense , High tensile strength, can completely replace allogeneic collagen membrane; 5) can be mixed with bone preparation or autologous bone, good p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/70A61K47/36A61K38/18A61P19/08
CPCA61K9/7007A61K38/18A61K47/36A61P19/08
Inventor 王丽萍肖殷万绵佳李正茂杜志斌宿玉成葛林虎
Owner STOMATOLOGY AFFILIATED STOMATOLOGY HOSPITAL OF GUANGZHOU MEDICAL UNIV
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