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A method for de-antigen nerve treatment

A treatment method and de-antigen technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve the problems that artificial materials cannot achieve natural nerve repair effect, donor site damage, etc., to increase biocompatibility and reduce cytotoxicity Effect

Active Publication Date: 2021-07-16
FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, artificial materials cannot achieve the repair effect of natural nerves. Autologous nerve transplantation usually causes damage to the donor site, and the source of the same kind of nerves is limited. Therefore, it is more advantageous to use xenogeneic nerves for damage repair

Method used

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  • A method for de-antigen nerve treatment
  • A method for de-antigen nerve treatment
  • A method for de-antigen nerve treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A method for de-antigen nerve treatment, comprising the steps of:

[0047] (1) Freshly isolated mammalian nerves are taken, and blood and impurities are washed with normal saline.

[0048] (2) Soak the nerve in sterile PBS buffer, shake at 100 rpm at 25°C for 5 hours.

[0049] (3) Put the nerve in the soaking solution, 20g nerve / 100ml soaking solution, carry out staged pressurization treatment at 4°C, and the staged pressurization treatment procedure is as follows:

[0050] 1) 1.5MPa, 20min;

[0051] 2) 10MPa, 5min;

[0052] 3) 25MPa, 2min.

[0053] The components of the soaking solution are as follows: 50 g / L of hydroxyethyl starch (molecular weight: 400,000 Da), 40 g / L of chondroitin sulfate, and 15 g / L of collagen, dissolved in water, and adjusted to pH 6.8-7.2.

[0054] (4) De-antigen treatment is carried out on the nerve after pressure treatment, and the specific steps are as follows:

[0055] 1) Soak the nerve in 2M NaCl solution overnight, and wash with ster...

Embodiment 2

[0061] A method for de-antigen nerve treatment, comprising the steps of:

[0062] (1) Freshly isolated mammalian nerves are taken, and blood and impurities are washed with normal saline.

[0063] (2) Soak the nerve in sterile PBS buffer, shake at 25°C, 70rpm, for 8h.

[0064] (3) Put the nerve in the soaking solution, 10g nerve / 100ml soaking solution, carry out staged pressurization treatment at 4°C, and the staged pressurization treatment procedure is as follows:

[0065] 1) 0.5MPa, 30min;

[0066] 2) 15MPa, 3min;

[0067] 3) 30MPa, 1min.

[0068] Replace with new soaking solution after treatment, and repeat the above procedure once.

[0069] The components of the soaking solution are as follows: 50 g / L of hydroxyethyl starch (molecular weight: 600,000 Da), 35 g / L of chondroitin sulfate, and 20 g / L of collagen, dissolved in water, and adjusted to pH 6.8-7.2.

[0070] (4) De-antigen treatment is carried out on the nerve after pressure treatment, and the specific steps are...

Embodiment 3

[0077] A method for de-antigen nerve treatment, comprising the steps of:

[0078] (1) Freshly isolated mammalian nerves are taken, and blood and impurities are washed with normal saline.

[0079] (2) Soak the nerve in sterile PBS buffer, shake at 25°C, 80rpm, for 6h.

[0080] (3) Put the nerves in the soaking solution, 15g nerves / 100ml soaking solution, and carry out staged pressure treatment at 4°C. The staged pressure treatment procedure is as follows:

[0081] 1) 1MPa, 25min;

[0082] 2) 12MPa, 4min;

[0083] 3) 28MPa, 1.5min.

[0084] The components of the soaking solution are as follows: hydroxyethyl starch (molecular weight: 150,000 Da) 60g / L, chondroitin sulfate 45g / L, collagen 10g / L, dissolved in water, and adjusted to pH 6.8-7.2.

[0085] (4) De-antigen treatment is carried out on the nerve after pressure treatment, and the specific steps are as follows:

[0086] 1) Soak the nerve in 2M NaCl solution overnight, and wash with sterile distilled water for 3 minutes;...

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Abstract

The invention discloses a treatment method for deantigenated nerves, which comprises the following steps: (1) taking fresh isolated nerves, washing blood and impurities with physiological saline; (2) immersing the nerves in sterile PBS buffer solution, shaking for 5 ‑8h; (3) Soak the nerve in the soaking solution containing hydroxyethyl starch and chondroitin sulfate, and perform staged pressure treatment for 1‑2 times; (4) De-antigenize the nerve after pressure treatment. The present invention effectively removes the antigenic substance attached to the nerve through the cooperation of various parameters in the process of PBS pretreatment, staged pressurization treatment and antigen removal treatment, ensures biocompatibility and reduces cytotoxicity.

Description

technical field [0001] The invention relates to the field of medical materials, and more specifically relates to a deantigenated nerve treatment method. Background technique [0002] Nerve damage usually results in a high disability rate. Therefore, clinically, a large number of high-quality nerve repair materials are needed to reconstruct and repair the damaged parts. However, artificial materials cannot achieve the repair effect of natural nerves. Autologous nerve transplantation usually causes damage to the donor site, and the source of the same kind of nerves is limited. Therefore, it is more advantageous to use xenogeneic nerves for damage repair. The deantigenation process of heterogeneous nerves often affects biocompatibility and increases cytotoxicity; therefore, how to improve the biocompatibility of nerves and reduce cytotoxicity during the deantigenation process has become an urgent problem to be solved by those skilled in the art. Contents of the invention [...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/50A61L27/36
CPCA61L27/3604A61L27/3675A61L27/3687A61L27/3691A61L27/50A61L2430/32A61L2430/40
Inventor 赵彦涛衷鸿宾侯树勋韩丽伟胡先同白玉龙张看李利张春丽章亚东
Owner FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA