In vitro simulated circulating tumor cell production device and application thereof

Abstract

The invention discloses an in vitro simulated circulating tumor cell production device, which comprises a culture dish and a sealing cover. The sealing cover is used for sealing the culture dish; theculture dish is divided into a first part and a second part by a separating device; the first part is a tumor cell culture department; the second part is a circulating tumor cell enrichment part; theseparating device is provided with a cell transfer site; the tumor cells from the first part can enter the second part through the cell transfer site; the second part is provided with a semipermeablemembrane ventilator window for ensuring adequate oxygen the second part. The device can rapidly and easily prepare circulating tumor cells in a large amount; and the prepared circulating tumor cells have similar characteristics as the circulating tumor cells obtained by animal models, and can be used for subsequent molecular and functional analysis of circulating tumor cells. The invention is of great significance for further research on circulating tumor cells.

Description

technical field [0001] The invention relates to an in vitro simulated circulation tumor cell generation device and its application. Background technique [0002] Malignant tumor metastasis is the main cause of death of tumor patients, and it is also the focus of current tumor research. In the process of tumor metastasis, tumor cells first break away from the primary tumor, invade and pass through the host stroma to enter the circulation, survive and reach the distant capillary bed, where they adhere and penetrate the blood vessels, enter the organ parenchyma, colonize and proliferate and grow into secondary cells. Tumors. [0003] In 1889, British doctor Stephen Paget expounded his "seed and soil theory", arguing that metastasis requires tumor cell dissemination, that is, "seed", and the ideal environment for receiving organs is "fertile soil", and the organ microenvironment ("soil" ) can affect the seeding, invasion, survival, growth of specific tumor cells ("seeds"). Se...

AI Technical Summary

Problems solved by technology

However, the technology is difficult and the cost is high

Due to the small number of circulating tumor cells in peripheral blood, it is difficult to separate and capture them. It is difficult to obtain a large number of circulating tumor cells for their molecular and functional analysis under the current technical conditions.

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