Evaluation method for antioxidative interactions
An evaluation method and interaction technology, applied in the field of chemical analysis, can solve the problems of inapplicable analysis, non-linear correlation of antioxidant activity, etc., and achieve the effect of simple calculation
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Embodiment 1
[0021] A method for evaluating the antioxidant interaction of blueberry 80% acidified methanol extract and mango acetone extract, comprising the following steps.
[0022] (1) Establishment of ABTS in vitro + ·Model.
[0023] Principle of model establishment: ABTS is oxidized into green ABTS under the action of appropriate oxidizing agent + , ABTS in the presence of antioxidants + The production of · will be inhibited, and the antioxidant capacity of the sample can be measured and calculated by measuring the absorbance of ABTS at 734nm.
[0024] Mix 5 mL of 7.4 mmol / LABTS stock solution with 88 µL of 2.6 mmol / L K 2 S 2 o 8 Mix the solution, let it stand for 12-16 h, and prepare ABTS working solution; the absorbance measured at a wavelength of 734 nm under normal temperature is 0.7±0.02; 0.2 mL of ABTS working solution is mixed with 10 µL of different concentrations of the test substance, and kept away from light at room temperature Stand still for 6 min, measure the absor...
Embodiment 2
[0034] A method for evaluating the antioxidant interaction of mulberry 80% acidified methanol extract and watermelon acetone extract, comprising the following steps.
[0035] (1) Establish an in vitro DPPH free radical model.
[0036] The principle of model establishment: DPPH free radical has a single electron and has a strong absorption at 517nm, and its alcohol solution is purple. When there is a free radical scavenger, its absorption gradually disappears due to pairing with its single electron, and its fading degree has a quantitative relationship with the number of electrons it accepts, so a spectrophotometer can be used for rapid quantitative analysis.
[0037] Model establishment method: 2 mL 1×10 -4 Mix the mol / L DPPH solution with an equal volume of the test substance with different mass concentrations, and shake well. The reaction was carried out at room temperature and under dark light for 30 min, and the absorbance was measured at a wavelength of 517 nm, and the...
Embodiment 3
[0047] A method for evaluating the antioxidant interaction of purple sweet potato 80% acidified methanol extract and carrot acetone extract comprises the following steps.
[0048] (1) Establish H 2 o 2 Induced H9c2 in vitro cellular oxidation model.
[0049] Model establishment method: H9c2 cells in the logarithmic phase were evenly planted in 96-well plates, and after 24 hours of culture, a certain concentration of agricultural products / agricultural product combination extracts (purple sweet potato, HP; carrot: lipophilicextracts of carrots) were added to each well. , LC) acted on H9c2 cells for 12 h, and the combination of purple potato and carrot 3:7 (m / m) was used to act on the cells, and the corresponding mixing ratio was HE-LC (F3 / 10). Subsequently, the establishment of H 2 o 2 Oxidation model, add 200 µL H to each well 2 o 2 (100 µmoL / L) complete culture solution, cultured for 1 h, washed with PBS, added 200 µL MTT (5g / L) to each well, reacted for 4 h, discarded t...
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