Living body single-cell in situ extraction and online mass spectrum detection device and application

A mass spectrometry detection and single-cell technology, which is applied in the field of single-cell mass spectrometry research, can solve the problems that the insertion position cannot be determined and cannot reflect all the information of single cells, etc., and achieve the effect of fast cell analysis

Active Publication Date: 2019-04-02
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the currently existing and widely used single-cell mass spectrometry technologies, the single-cell droplet technology is difficult to avoid experimental errors due to the low single-cell loading rate; Unable to determine, unable to reflect all the information of the entire single cell

Method used

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  • Living body single-cell in situ extraction and online mass spectrum detection device and application
  • Living body single-cell in situ extraction and online mass spectrum detection device and application

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Such as figure 1 with figure 2 As shown, a living single cell in situ extraction and online mass spectrometry detection device, which includes:

[0049] A microfluidic platform, which includes a peristaltic syringe pump 1 and a fluid pipeline, the fluid pipeline includes a first fluid pipeline 2-1 and a second fluid pipeline 2-2, and the peristaltic syringe pump is used to drive the extraction in the fluid pipeline a fluid to deliver the extraction fluid to the single cell probe;

[0050] A cell manipulation platform comprising a stage ( figure 1 not shown in), placed on the petri dish 3 on the stage, cell culture fluid is filled in the petri dish 3, the cells to be detected are cultivated in the petri dish, and a microscope objective lens 4 is arranged below the stage, which is used for Observe and locate the cells to be detected;

[0051] The single-cell probe located above the cell manipulation platform includes a fluid inlet 11, a single-cell extraction chamber...

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Abstract

The invention provides a living body single-cell in situ extraction and online mass spectrum detection device and application. The device comprises a microfluidic platform, a cell operation platform,a single-cell probe, an online ionization device and a detection device, wherein the microfluidic platform is used for transmitting extracted fluid to the single-cell probe; cells to be detected are cultured on the cell operation platform; the single-cell probe is positioned on the top of the cell operation platform for isolating a target single cell to be detected and realizing single-cell extraction to obtain a single-cell extraction object; the online ionization device is used for receiving the single-cell extraction object and forming ionization spraying mist for the single-cell extractionobject; and the detection device is used for receiving and detecting the ionization spraying mist formed by the online ionization device. When the device is used for carrying out cell detection, after the extracted cell detects the marker of the cell, protein and nucleic acid information in the cell can be kept to a maximum degree for subsequent analysis.

Description

technical field [0001] The invention belongs to the technical field of single-cell mass spectrometry research, and relates to an in-situ extraction and online mass spectrometry detection device for living single cells and its application. Background technique [0002] Single-cell analysis technology combines microfluidic technology with electrochemical analysis, fluorescence analysis, mass spectrometry and other technologies to analyze cell markers, metabolites, genome and proteome at the single-cell level. With the advancement of analytical methods, more and more single-cell detection technologies have emerged. Because single-cell analysis can provide important information for disease diagnosis and gene sequencing with low consumption, it has attracted wide attention. [0003] Mass spectrometry, as an important means of single-cell chemical composition analysis, has the characteristics of high sensitivity, high throughput and simultaneous analysis of multiple important com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N1/28G01N1/34C12M3/00C12M1/00
CPCG01N1/28G01N1/34G01N27/62C12M23/16
Inventor 林金明黄秋实毛思锋
Owner TSINGHUA UNIV
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