Quick identification method for salt tolerance of linseed variety in germination period
An identification method and technology of salt tolerance, applied in the fields of seed and rhizome treatment, instrument, data processing application, etc., can solve the problem of time-consuming and laborious, and achieve the effect of high accuracy
Active Publication Date: 2019-04-05
甘肃省农业科学院作物研究所
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Problems solved by technology
The identification work usually needs to measure many indicators, and the results obtained after comprehensive analysis can be more realistic, so the identification work will inevitably be time-consuming and laborious
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Embodiment 1
[0020] 1. a kind of method for rapid identification of flax variety germination stage salt tolerance, the steps are as follows:
[0021] (1) Seed germination: select flax seeds with full grains and the same size for each variety, sterilize them with 70% ethanol solution for 1 minute, then rinse them with sterile water for 4-5 times, dry the water on the surface of the seeds, and place them on the floor There are 2 layers of filter paper in a petri dish with a diameter of 9 cm, and 30 seeds are placed in each dish. Add 15ml 100mmol·L to each petri dish -1 The NaCl solution of the flax species was used to stress the seeds of the flax variety, and 15ml of distilled water was added to the control, and the salt treatment and the control were repeated three times. After the corresponding marks were made, they were placed in a dark place in a constant temperature tissue culture room at 25°C for germination.
[0022] (2) Index measurement and statistics: Count the number of germinati...
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The invention provides a quick identification method for salt tolerance of a linseed variety in the germination period. The method comprises the following steps that (1), an NaCl solution of a certainconcentration is adopted for conducting stress on seeds of the linseed variety, and distilled water is taken as a control group; the number of sprouts is counted from the third day of germination tothe seventh day; after seven days of germination, the length of the hypocotyledonary axis, the fresh weight and the dry weight of the radicle are measured; (2), the germination index and a relative value are calculated; (3), the germination index and the relative value are substituted into a regression equation, the Z value of the linseed variety is calculated, and the salt tolerance is judged according to the Z value. According to the method, the NaCl solution of the certain concentration is adopted for conducting stress on the seeds of the linseed variety, four key indexes significantly related with the salt tolerance are measured, the regression equation is utilized, and therefore identification evaluation for the salt tolerance of the tested material can be quickly conducted in the germination period. The method is verified by a comprehensive analysis method, achieves significant correlation and has higher accuracy; it is not necessary to measure multiple indexes in the aspect of identification work.
Description
technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a rapid identification method for the salt tolerance of flax varieties in the germination stage. Background technique [0002] Flax (linseed for oil) belongs to the genus Linum of the family Lianceaceae. Flax seeds are rich in lignans, soluble plant cellulose, and α-linolenic acid rich in flax oil and various unsaturated fatty acids necessary for human body. etc., have good health care functions for the human body, have important utilization value, and are also the main source of edible oil for the people in my country's industrial dry vegetable oil and production areas. They are widely used in Gansu, Inner Mongolia, Ningxia, Shanxi, Hebei and other provinces and regions to plant. At present, the impact of soil salinization on agricultural production has become the second largest abiotic stress factor after drought. At present, there are about 1 billion hm in t...
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IPC IPC(8): A01C1/00G06Q10/06G06Q50/02
CPCA01C1/00G06Q10/06393G06Q50/02
Inventor 赵玮张艳萍张建平王利民赵利王斌党照李闻娟谢亚萍齐燕妮
Owner 甘肃省农业科学院作物研究所