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A rapid identification method for salt tolerance of flax varieties during germination

An identification method and technology of salt tolerance, applied in the field of rapid identification of salt tolerance of flax varieties during germination, can solve problems such as time-consuming and laborious, and achieve the effect of high accuracy

Active Publication Date: 2021-11-05
甘肃省农业科学院作物研究所
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Problems solved by technology

The identification work usually needs to measure many indicators, and the results obtained after comprehensive analysis can be more realistic, so the identification work will inevitably be time-consuming and laborious

Method used

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  • A rapid identification method for salt tolerance of flax varieties during germination
  • A rapid identification method for salt tolerance of flax varieties during germination
  • A rapid identification method for salt tolerance of flax varieties during germination

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Embodiment 1

[0020] 1. a kind of method for rapid identification of flax variety germination stage salt tolerance, the steps are as follows:

[0021] (1) Seed germination: select flax seeds with full grains and the same size for each variety, sterilize them with 70% ethanol solution for 1 minute, then rinse them with sterile water for 4-5 times, dry the water on the surface of the seeds, and place them on the floor There are 2 layers of filter paper in a petri dish with a diameter of 9 cm, and 30 seeds are placed in each dish. Add 15ml 100mmol·L to each petri dish -1 The NaCl solution of the flax species was used to stress the seeds of the flax variety, and 15ml of distilled water was added to the control, and the salt treatment and the control were repeated three times. After the corresponding marks were made, they were placed in a dark place in a constant temperature tissue culture room at 25°C for germination.

[0022] (2) Index measurement and statistics: Count the number of germinati...

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Abstract

The invention provides a quick identification method for the salt tolerance of flax varieties in the germination stage, comprising the following steps: (1) using a certain concentration of NaCl solution to stress the seeds of flax varieties, and using distilled water as a control group; counting from the 3rd day of germination The number of germination is counted until the 7th day; after 7 days of germination, measure the hypocotyl length, total fresh weight and radicle dry weight; (2) calculate the germination index and relative value; (3) substitute into the regression equation to calculate the Z value and judge the salt tolerance according to the Z value. The present invention mainly uses a certain concentration of NaCl solution to stress the flax variety seeds, measures four key indicators significantly related to salt tolerance, and uses a regression equation to quickly identify and evaluate the salt tolerance of the test materials in the germination period. This method has been verified by a comprehensive analysis method, reaching extremely significant correlation, and has high accuracy; at the same time, there is no need to measure many indicators in the identification work.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a rapid identification method for the salt tolerance of flax varieties in the germination stage. Background technique [0002] Flax (linseed for oil) belongs to the genus Linum of the family Lianceaceae. Flax seeds are rich in lignans, soluble plant cellulose, and α-linolenic acid rich in flax oil and various unsaturated fatty acids necessary for human body. etc., have good health care functions for the human body, have important utilization value, and are also the main source of edible oil for the people in my country's industrial dry vegetable oil and production areas. They are widely used in Gansu, Inner Mongolia, Ningxia, Shanxi, Hebei and other provinces and regions to plant. At present, the impact of soil salinization on agricultural production has become the second largest abiotic stress factor after drought. At present, there are about 1 billion hm in t...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00G06Q10/06G06Q50/02
CPCA01C1/00G06Q10/06393G06Q50/02
Inventor 赵玮张艳萍张建平王利民赵利王斌党照李闻娟谢亚萍齐燕妮
Owner 甘肃省农业科学院作物研究所