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A bio-ink with uniform cell distribution based on 3D printing and its assembly method

A bio-ink, 3D printing technology, applied in prosthesis, additive processing, medical science and other directions, can solve the problem of uneven cell distribution, achieve the effect of slowing down cell deposition, facilitating printing and maintaining biocompatibility

Active Publication Date: 2021-02-26
深圳大洲医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is that during the 3D printing process, the target cells in the bio-ink will be deposited at the bottom of the barrel, resulting in uneven distribution of cells in the printed tissue structure

Method used

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  • A bio-ink with uniform cell distribution based on 3D printing and its assembly method
  • A bio-ink with uniform cell distribution based on 3D printing and its assembly method
  • A bio-ink with uniform cell distribution based on 3D printing and its assembly method

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Experimental program
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Effect test

Embodiment 1

[0051] The bioink body A of the embodiment of the present invention is made up of the following mass fraction components:

[0052] Methacrylated Gelatin 7%;

[0053] Silk protein microspheres 0.5%, particle size 0.1mm;

[0054] Fetal bovine serum 1%;

[0055] Photoinitiator 2959 0.1%;

[0056] HEPES Equilibrium Buffer 91.6%, pH=7.2.

[0057] The preparation method of the bio-ink body A of the embodiment of the present invention is as follows: dissolve the methacrylic anhydride gelatin in the HEPES equilibrium buffer solution according to the above ratio, add photoinitiator 2959, and stir at 50°C to obtain a uniformly mixed solution. Filter and sterilize with PES membrane filter, then cool down to 37°C, finally add silk protein and fetal bovine serum, and mix evenly by ultrasonic.

[0058] Will 1╳10 6 A bioactive cell was added to the prepared bio-ink body A and mixed evenly to obtain bio-ink A.

Embodiment 2

[0060] The bioink body B of the embodiment of the present invention is made up of the following mass fraction components:

[0061] Methacrylated Gelatin 7%;

[0062] Silk protein microspheres 0.75%, particle size 0.1mm;

[0063] Fetal bovine serum 1%;

[0064] Photoinitiator 2959 0.1%;

[0065] HEPES Equilibrium Buffer 91.15%, pH=7.2.

[0066] The preparation method of the bio-ink body B of the embodiment of the present invention is the same as that of embodiment 1.

[0067] Will 1╳10 6 A bioactive cell was added to the prepared bio-ink body B and mixed evenly to obtain bio-ink B.

Embodiment 3

[0069] The bioink body C of the embodiment of the present invention is made up of the following mass fraction components:

[0070] Methacrylated Gelatin 7%;

[0071] Silk protein microspheres 1%, particle size 0.1mm;

[0072] Fetal bovine serum 1%;

[0073] Photoinitiator 2959 0.1%;

[0074] HEPES Equilibrium Buffer 90.9%, pH=7.2.

[0075] The preparation method of the bio-ink body C of the embodiment of the present invention is the same as that of the embodiment 1.

[0076] Will 1╳10 6 A bioactive cell is added to the prepared bio-ink body C and mixed evenly to obtain bio-ink C.

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Abstract

The invention discloses a bio-ink based on 3D printing with uniform distribution of cells, which includes a bio-ink body and bioactive cells suspended in the bio-ink body, and the bio-ink body includes the following components: methacrylic anhydride Gelatin, silk protein microspheres, fetal bovine serum, photocrosslinking initiator, equilibrating buffer solution. The bio-ink of the technical solution of the present invention increases the viscosity of the bio-ink by adding silk protein microspheres, increases the buoyancy of the bioactive cells in the bio-ink, slows down the sinking of cells at all levels of the bio-ink, and improves the bio-3D printing process. Cell deposition phenomenon, each component is water-soluble, has good biocompatibility, does not have cytotoxicity, contains biologically active components, and can be biodegraded, in complex, fine bioprinting and composite cell bioprinting It has a good application prospect.

Description

technical field [0001] The invention relates to a biological ink, in particular to a 3D printing-based biological ink with uniform distribution of cells and an assembly method thereof. Background technique [0002] With the advancement of biomaterial technology, 3D bioprinting, as an emerging technology, has high application prospects in the field of tissue engineering because of its good biocompatibility and the ability to achieve bioregenerative repair. [0003] Among them, bio-ink, bio-printing technology and target biological cells have been extensively and in-depth researched as the three major elements of bio-3D printing. Due to the diversity, complexity and precision of human tissue structure, the 3D bioprinting process of its bionic structure takes a long time, and the target cells in the bio-ink will fall and deposit at the bottom of the barrel due to the action of gravity, resulting in the printed tissue The cells are unevenly distributed in the structure. Conte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/38B33Y70/10
CPCA61L27/222A61L27/227A61L27/3804B33Y70/00
Inventor 朱铠孙晓宁陈楠张炜佳程蕾蕾王春生
Owner 深圳大洲医学科技有限公司
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