Spiral microchannel and use method thereof, and series-parallel installation structure

Abstract

The invention belongs to the field of rare cell or particle enrichment and screening, and relates to a spiral microchannel and a use method thereof, and a series-parallel installation structure. An inertial separation structure of a series-parallel spiral microchannel with reasonable cascading and application of multiple spiral microchannels are skillfully designed for the technical problem of thespiral microchannel in the field of sorting and enrichment of peripheral blood circulating tumor cells in a patient with a malignant tumor. Compared with the existing similar technology, the presentinvention not only has a good collection efficiency and processing flux of circulating tumor cells, but also maximizes the purity of circulating tumor cells and minimizes the loss of circulating tumorcells, and maintains the original phenotype of circulating tumor cells for sorting and enriching peripheral blood circulating tumor cells. The obtained cell suspension containing high-purity circulating tumor cells is particularly suitable for subsequent biomedical detection, genetic analysis, cell culture, xenograft tumor preparation, etc., and has extensive and far-reaching clinical practical application value.

Description

Technical field [0001] The invention belongs to the field of rare cell or particle enrichment screening, and relates to a spiral microchannel, a use method thereof, and a series and parallel installation structure. Background technique [0002] Invasion and metastasis are the main clinical causes of death in tumor patients. Deaths caused by tumor metastasis diseases account for about 90% of deaths caused by solid tumors. Therefore, early and accurate detection of tumor metastasis appears to be extremely important. Circulating tumor cells refer to tumor cells that break away from primary and metastatic tumor lesions, invade and enter the circulatory system such as lymphatic vessels and blood. The detection of circulating tumor cells in the circulatory system suggests that there may be tumor micrometastasis, and the possibility of further developing into distant metastasis. In recent years, many research reports have confirmed that circulating tumor cells have potentially importan...

AI Technical Summary

Problems solved by technology

but, Existing helical inertial microfluidic chips The main disadvantage of the single-channel helical microchannel inertial microfluidic chip is the low purity of the obtained circulating tumor cells, which leads to great difficulties in the subsequent identification and clinical application of circulating tumor cells. lower value

Although the existing spiral microchannels still have major deficiencies and related technical bottlenecks in sorting and enriching circulating tumor cells, the existing research results suggest that if multiple single The spiral microchannel can greatly improve the purity of circulating tumor cells in the sorted and enriched cell mixture under the premise of ensuring good capture efficiency and processing throughput, so that it can meet the actual clinical needs

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