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Bifidobacterium fermentation method of pollen and application of fermentation product of pollen

A technology of bifidobacteria and fermentation method, applied in the application, lactobacillus, bacteria used in food preparation, etc., can solve the problem that the solubility is not greatly improved, the nutritional value and added value are not high, and the taste of pollen is not solved. and other problems to achieve the effect of improving flavor and taste, enriching varieties, and improving nutritional value

Inactive Publication Date: 2019-04-09
广州欧普康特医食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, although mechanical or enzymatic wall breaking improves the solubility of pollen, it does not solve the taste of pollen. There are also probiotics used to ferment pollen, which improves the taste of pollen, but the solubility has not been greatly improved. improvement
Pollen products on the market are relatively simple, mainly broken wall pollen, broken wall pollen flakes, the nutritional value and added value of the product are not high

Method used

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  • Bifidobacterium fermentation method of pollen and application of fermentation product of pollen

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1) After removing the sundries of pollen, ultrafinely pulverize to 200 mesh, and sterilize by ultraviolet light for 30 minutes;

[0042] 2) Prepare pure water according to the weight of pollen at 1:5, and then sterilize;

[0043] 3) Putting the pollen and sterilized water into a colloid mill and grinding to obtain a pollen homogenate;

[0044] 4) Inoculate the bifidobacterium into the pollen homogenate according to the ratio of 5% inoculum amount, and ferment for 12 hours at 30° C. and 100 r / min;

[0045] 5) After the fermentation, add 10% trehalose and 20% maltodextrin to the fermentation product as protective agents, mix and dissolve; freeze-dry at a vacuum of 5Pa and a temperature of -50°C to reduce the moisture to 5% Below, that is.

Embodiment 2

[0047] 6) After removing the sundries of pollen, sterilize by ultraviolet light for 20 minutes;

[0048] 7) Prepare pure water according to 1:10 of pollen weight, and then sterilize;

[0049] 8) Putting pollen and sterilized water into a colloid mill and grinding to obtain pollen homogenate;

[0050] 9) Add the bifidobacterium powder into the pollen homogenate according to the ratio of 1% inoculum amount, and ferment for 36 hours at 40° C. and 180 r / min.

[0051] 10) After the fermentation, add 20% trehalose and 20% maltodextrin to the fermentation product as protective agents, mix and dissolve; freeze-dry at a vacuum degree of 15Pa and a temperature of -60°C to reduce the moisture content to 5% Below, that is.

Embodiment 3

[0053] 1) After removing pollen debris, sterilize with ultraviolet light for 30 minutes;

[0054] 2) Prepare pure water according to 1:8 of pollen weight, and then sterilize;

[0055] 3) Putting the pollen and sterilized water into a colloid mill and grinding to obtain a pollen homogenate;

[0056]4) Add the bifidobacterium powder into the pollen homogenate according to the ratio of 5% inoculum amount, and ferment for 24 hours at 40°C and 180r / min;

[0057] 5) After the fermentation, add 15% trehalose and 15% maltodextrin to the fermentation product as protective agents, mix and dissolve; freeze-dry at a vacuum of 15Pa and a temperature of -60°C to reduce the moisture to 5% Below, that is.

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Abstract

The invention provides a bifidobacterium fermentation method of pollen and application of a fermentation product of the pollen. According to the method, primary broken wall treatment is carried out onthe pollen by utilizing a colloidal mill and ultrafine grinding, and then the ground pollen is fermented by bifidobacteria, the probiotic content in the fermentation product is 10<9> cfu / g or more, and the probiotic content in a solid beverage prepared from the fermentation product is 10<8> cfu / g or more.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a pollen bifidobacteria fermentation method and its fermentation product and application. Background technique [0002] As the essence of plants, pollen was recognized and utilized by our ancestors as early as 2,000 years ago. Pollen is rich in nutrients. In addition to protein, amino acid, multivitamins, enzymes, trace elements, biologically active substances, etc., in addition to the nutrients needed by the human body, it also contains a variety of substances that have special functions on human physiological functions. For example, flavonoids for anti-oxidation and anti-radiation, biologically active enzymes for delaying aging and restoring fatigue, unsaturated fatty acids for promoting growth and treating cardiovascular diseases, dietary fiber for lowering blood sugar, etc. [0003] Because pollen has a hard cell wall, the sporopollenin in its structure has ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/38A23L2/39A23L33/00A23L5/00
CPCA23L2/382A23L2/39A23L5/00A23L33/00A23V2002/00A23V2400/125A23V2400/123A23V2400/113A23V2400/165A23V2200/30A23V2250/5114A23V2250/636A23V2250/6422A23V2300/10
Inventor 罗楚波
Owner 广州欧普康特医食品有限公司