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Small dense low-density lipoprotein cholesterol assay kit and application thereof

A low-density lipoprotein and kit technology, which is applied in the field of small and dense low-density lipoprotein cholesterol determination kits, can solve problems such as poor accuracy, and achieve the effects of improving accuracy, reducing interference and simple components

Inactive Publication Date: 2019-04-12
浙江夸克生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the defects of poor accuracy existing in the determination process of sdLDL-C content in the prior art, the present invention provides a small and dense low-density lipoprotein cholesterol determination kit, which has high accuracy, high specificity and wide Linear range, suitable for clinical full-automatic or semi-automatic biochemical analysis

Method used

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  • Small dense low-density lipoprotein cholesterol assay kit and application thereof
  • Small dense low-density lipoprotein cholesterol assay kit and application thereof
  • Small dense low-density lipoprotein cholesterol assay kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The components and concentrations of the small and dense low-density lipoprotein cholesterol assay kit of the present embodiment are as follows:

[0051] Reagent R1 includes: cholesterol esterase 1000.0U / L, cholesterol oxidase 1000.0U / L, phospholipase 10.0KU / L, catalase 1000.0KU / L, 2,4,6-tribromo-3-hydroxybenzoic acid 0.5mmol / L, 3-morpholine propanesulfonic acid buffer solution 50.0mmol / L with a pH value of 7.0 and chitin 0.2g / L;

[0052] Reagent R2 includes: peroxidase 5000.0U / L, 4-aminoantipyrine 1.0mmol / L, sodium azide 0.05mL / L and 3-morpholine propanesulfonic acid buffer solution with a pH value of 7.0 50.0mmol / L L.

Embodiment 2

[0054] The components and concentrations of the small and dense low-density lipoprotein cholesterol assay kit of the present embodiment are as follows:

[0055] Reagent R1 includes: cholesterol esterase 3000.0U / L, cholesterol oxidase 3000.0U / L, phospholipase 50.0KU / L, catalase 3000.0KU / L, 2,4,6-tribromo-3-hydroxybenzoic acid 5.0mmol / L, 3-morpholine propanesulfonic acid buffer solution 150.0mmol / L with a pH value of 7.0 and chitin 2.5g / L;

[0056] Reagent R2 includes: peroxidase 7000.0U / L, 4-aminoantipyrine 5.0mmol / L, sodium azide 0.5mL / L and 3-morpholine propanesulfonic acid buffer solution with a pH value of 7.0 150.0mmol / L L.

Embodiment 3

[0058] The components and concentrations of the small and dense low-density lipoprotein cholesterol assay kit of the present embodiment are as follows:

[0059] Reagent R1 includes: cholesterol esterase 1500.0U / L, cholesterol oxidase 1200.0U / L, phospholipase 20.0KU / L, catalase 1500.0KU / L, 2-hydroxy-3-m-toluidine propanesulfonate 1.0 mmol / L, borate buffer solution 75.0mmol / L with a pH value of 7.0 and chitin 0.5g / L;

[0060] Reagent R2 includes: peroxidase 5500.0U / L, 4-aminoantipyrine 1.3mmol / L, thimerosal 0.1mL / L and borate buffer solution with a pH value of 7.0 75.0mmol / L.

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Abstract

The invention relates to a small dense low-density lipoprotein cholesterol measuring kit, and belongs to the technical field of biological detection. The kit is composed of a reagent R1 and a reagentR2, wherein the reagent R1 includes cholesterol esterase, cholesterol oxidase, phospholipase, catalase, a color developing reagent and a buffer, the reagent R2 includes peroxidase, 4-aminoantipyrine,a preservative and a buffer, and the reagent R1 further includes chitin. The kit adopts a peroxidase method for determination and has the advantages of high accuracy, high specificity and wide linearrange.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a small and dense low-density lipoprotein cholesterol assay kit and its application. Background technique [0002] Lipoproteins in human serum are mainly divided into chylomicrons (chylomicrons, CM), very-low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL) , low-density lipoprotein (low-density lipoprotein, LDL) and high-density lipoprotein (high-density lipoprotein, HDL). [0003] Cholesterol, also known as cholesterol, exists in blood lipoproteins in the form of free cholesterol or cholesterol ester bound to fatty acids, such as: small and dense low-density lipoprotein cholesterol (sdLDL-C), very low-density lipoprotein cholesterol (VLDL-C), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), etc. [0004] Fishe proposed for the first time that low-density lipoprotein particles have polydispers...

Claims

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Application Information

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IPC IPC(8): C12Q1/60C12Q1/44C12Q1/30C12Q1/28C12Q1/26
Inventor 陈青松林耀文
Owner 浙江夸克生物科技有限公司