Rapid and efficient method for induction and propagation of bryophyllum hairy roots
A technology of rooting on the ground and hairy roots, applied in the field of agricultural biotechnology research, can solve the problems of long growth cycle and low transformation efficiency, and achieve the effects of fast analysis speed, simple operation, and simple and intuitive method
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Embodiment 1
[0022] Embodiment 1: a kind of method for fast and efficiently inducing big leaves to fall to the ground and take root hairy roots, comprising steps:
[0023] (1) Infection solution A: obtained by centrifuging 50 ml of A4 Agrobacterium with an OD600 value of 0.8 and resuspending it with an equal volume of MS liquid, and mixing it for later use;
[0024] (2) Infection solution B: 50 ml of A4 Agrobacterium with a GUS reporter gene with an OD600 value of 0.8 was centrifuged and resuspended with an equal volume of MS liquid, and mixed for later use;
[0025] (3) Take two bottles of 2-week-old big leaves to take root, cut them into sections, add one group to 50 ml of infecting solution A, completely soak the explants in the liquid, and infect for 5-10 minutes. The other group was added to 50 ml of infection solution B, and infected for 5-10 minutes;
[0026] (4) Place on solid medium without anti-MS, and culture in dark at 25 °C for 48 hours;
[0027] (5) After co-cultivation, tr...
Embodiment 2
[0031] Embodiment 2: a kind of method for fast and efficiently inducing bar leaves to take root hairy roots, comprising steps:
[0032] (1) Infection solution A: obtained by centrifuging 50 ml of A4 Agrobacterium with an OD 600 value of 0.8 and resuspending it with an equal volume of MS liquid, and mixing it for later use.
[0033] (2) Infection solution B: 50 ml of A4 Agrobacterium with a GUS reporter gene with an OD 600 value of 0.8 was centrifuged and resuspended with an equal volume of MS liquid, and mixed well for later use.
[0034] (3) Take two bottles of 2-week-old stick leaves to take root, and cut them into sections. One group was added to 50 ml of infection solution A, so that the explants were completely immersed in the solution, and infected for 5-10 minutes. Another group was added to 50 ml of infection solution B, and infected for 5-10 minutes.
[0035] (4) Place on MS solid medium and culture in dark at 25 °C for 48 hours.
[0036] (5) After co-cultivation, ...
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