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This invention discloses primers, kits, methods, and applications for detecting pathogenic *Pseudomonas proteus* strains based on the *oprL* gene, belonging to the field of microbial detection technology. This invention provides specific primers for detecting pathogenic *P. proteus* strains based on the *oprL* gene. The primers are designed based on the differential patterns identified after multiple sequence alignment, specifically by analyzing the results of multiple sequence alignments of all *P. proteus* *oprL* gene sequences in the NCBI database. The nucleotide sequences of the primers are shown in SEQ ID No. 1, SEQ ID No. 2, and SEQ ID No. 3. The *oprL* gene of *P. proteus* is amplified by PCR, and the PCR amplification products are then subjected to electrophoresis. The pathogenicity of *P. proteus* can be effectively distinguished based on the electrophoresis image, enabling rapid detection of *P. proteus* infecting large yellow croaker. The primers have good specificity, and the detection method is simple and intuitive.

The invention discloses a primer, a kit and a method for detecting pathogenic pseudomonas proteinans based on a pseudomonas proteinans strain oprL gene and application, and belongs to the technical field of microbiological detection. The invention provides a specific primer for detecting pathogenic pseudomonas deformation based on pseudomonas deformation strain oprL genes, and the specific primer is designed according to a difference rule found after multi-sequence comparison, namely, a PCR amplification primer of the oprL genes is designed according to a result obtained after multi-sequence comparison and arrangement of all the pseudomonas deformation strain oprL genes in an NCBI database, and the specific primer is used for detecting the pathogenic pseudomonas deformation based on the oprL genes of the pseudomonas deformation strain oprL genes. The nucleotide sequences of the primers are as shown in SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3. The oprL gene of pseudomonas proteinatica is amplified through PCR (polymerase chain reaction), then the PCR amplification product is subjected to electrophoresis, whether pseudomonas proteinatica has pathogenicity or not can be effectively distinguished according to the imaging condition of an electrophoretogram, rapid detection of pseudomonas proteinatica infected with pseudosciaena crocea can be realized, the specificity of the primers is good, and the detection method is simple, convenient and visual.