SSR molecular marker for identifying hybrid of Xinyu grape and Kyoho grape and application thereof

Abstract

The invention provides an SSR molecular marker for identifying a hybrid of Xinyu grape and Kyoho grape. The SSR molecular marker includes 5 pairs of SSR marker primers VMC7h3, Scu15vv, Vchr13a, UDV-088 and VrZAG67. The invention also provides application of the marker. The application includes conducting DNA extraction of the paternal Kyoho grape, the maternal Xinyu grape and the hybrid of the Xinyu grape and the Kyoho grape, establishing a PCR reaction system and amplification reaction procedure, conducting electrophoretic separation of PCR products and identifying the authenticity of the hybrid by using the 5 pairs of SSR-labeled primers in sequence. The 5 pairs of SSR labeled primers screened in the earlier stage are utilized to identify the hybrid of the Xinyu grape and the Kyoho grape, the true hybrid can be identified, the hybridization effect of the Xinyu grape and the Kyoho grape can be tested quickly, and the fake hybrid later generations can be eliminated in advance. The breeding efficiency is improved, and the cost is saved.

Description

[0001] The invention belongs to the technical field of identification of hybrids, and in particular relates to an SSR molecular marker for identifying hybrids of Xinyu grape and Kyoho grape and its application. Background technique [0002] Grape breeding mainly includes methods such as hybridization, seed selection, budding, and mutation. Hybrid breeding is an important way of grape breeding. This method is to obtain a new variety through the combination of gametes of two different genotype varieties under artificial control. In this way, multiple target traits can be combined to meet the comprehensiveness of modern breeding goals, such as resistance to stress (disease, drought, cold), seedless, fragrant seedless, high-yield fragrant and large grains, etc. Most of the grape varieties are hermaphroditic flowers, which can be self-fertilized or cross-pollinated. To carry out the hybridization work, the stamens of the female parent flower must be removed first, and the interfe...

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Problems solved by technology

[0003] Xinyu grape is a red, large-grained mid-to-late-maturing variety that can be used for both dry and fresh food. It has strong disease resistance, and it is expected to produce large-grained, dark-colored, fragrant, and highly resistant offspring by preparing the Xinyu × Kyoho hybrid combination, but the two varieties belong to the hybrid between diploid and tetraploid, and the grape heterozygosity High, easy to self-pollinate and bear fruit, the authenticity of hybridization of offspring needs to be verified

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