Real-time quantitative LAMP primer set and kit for detecting sheep mycoplasma pneumoniae

Abstract

The invention discloses a real-time quantitative LAMP primer set and a kit for detecting sheep mycoplasma pneumoniae, wherein the LAMP primer set is shown as SEQ ID NO: 1-5. The LAMP primer set is used for preparing an LAMP amplification kit for detecting the mycoplasma ovis, and the LAMP detection method provided by the kit has high specificity and sensitivity, good repeatability and high reliability, and specifically detects a specific nucleotide fragment of the mycoplasma ovis, and can carry out quantitative detection on the copy number of the mycoplasma sheep pneumoniae, quickly and accurately obtain a detection result, bring convenience to the simple and rapid detection of the mycoplasma sheep pneumoniae. The real-time quantitative LAMP primer set and the kit for detecting sheep mycoplasma pneumoniae solve the defects of time consumption, labor consumption and the like of the traditional identification method, are suitable for being used at a basic level, can be used as a rapid, accurate and simple detection tool for rapid detection and identification of mycoplasma ovis in a laboratory.

Description

technical field [0001] The invention relates to the technical field of microbial detection, in particular to a LAMP primer set and a kit for rapid and real-time quantitative detection of Mycoplasma pneumoniae. Background technique [0002] Ovine infectious pleuropneumonia is a chronic respiratory infectious disease of sheep caused by Mycoplasma ovipneumoniae (MO), which mainly harms lambs aged 1-3 months. characteristic pneumonia. The disease occurs in many countries in the world and is one of the main infectious diseases that harm sheep. MO was first isolated by Mackay et al. in 1963, and then Cottw found it in Australian sheep disease lung. Carmicheal et al. proved the pathogenicity of this mycoplasma, and proposed to name it Mycoplasma ovis pneumoniae. The Sichuan Institute of Animal Husbandry and Veterinary Medicine first discovered that the disease was brought in by Les special sheep imported from New Zealand and Australia in Aba, Sichuan in 1979. After isolation and ...

AI Technical Summary

Problems solved by technology

The conventional method is the traditional isolation and identification of the pathogen, that is, through the identification of the phenotypic characteristics and biochemical indicators of the pathogen. Mycoplasma ovis pneumoniae has high nutritional requirements on the medium, and the isolation rate is low. The conventional identification method is time-consuming and laborious.

Although the polymerase chain reaction (PCR) method has high specificity and sensitivity, it requires expensive PCR machines, electrophoresis tanks and other precision instruments, and is not suitable for grassroots development.

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