A kind of regenerated cellulose-myofibrillar protein gel and preparation method thereof
A technology of myofibrillar protein and regenerated cellulose, which is applied to regenerated cellulose-myofibrillin gel and its preparation, uses regenerated cellulose to improve the quality of pork myofibrillar protein gel, and can solve the problem of poor water retention of gel , low hardness, degradation of gel quality and other problems, to achieve the effect of improving water retention, improving texture characteristics, and improving quality
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Embodiment 1
[0028] Example 1: Myofibrillar protein gel
[0029] Step (1), the extraction of myofibrillar protein: remove pork hind leg connective tissue and fat, cut into small pieces and grind; Add 400mL standard salt solution (0.1mol / L NaCl, 20mmol / L Na 2 HPO 4 / NaH 2 PO 4 , 2mmol / L MgCl 2 , 1mmol / L EGTA, pH value 6.5), after homogenization, centrifuge at 2000g for 15min, repeat the homogenization and centrifugation twice; Then centrifuge at 2000g for 15min to obtain myofibrillar protein;
[0030] Step (2), the preparation of myofibrillar protein gel: adopt phosphate buffer solution (0.6mol / LNaCl, 50mmol / LNaCl 2 HPO 4 / NaH 2 PO 4 , pH=6.5) to prepare a solution with a myofibrillar protein concentration of 40mg / mL, homogenize at 6000rpm for 30s in an ice bath, repeat 3 times to ensure thorough mixing; take the mixed sample and place it in a 50mL centrifuge tube, and place it in a 2000g Centrifuge at a rotating speed for 5 minutes to remove air bubbles, then put the mixed sampl...
Embodiment 2
[0031] Example 2: Regenerated cellulose-myofibrillar protein gel
[0032] Step (1), the extraction of myofibrillar protein: remove pork hind leg connective tissue and fat, cut into small pieces and ground; Add 400mL standard salt solution (0.1mol / L NaCl , 20mmol / L Na 2 HPO 4 / NaH 2 PO 4 , 2mmol / L MgCl 2 , 1mmol / L EGTA, pH value 6.5), after homogenization, centrifuge at 2000g for 15min, repeat the homogenization and centrifugation twice; Then centrifuge at 2000g for 15min to obtain myofibrillar protein;
[0033] Step (2), preparation of regenerated cellulose: Wet 3 g of microcrystalline cellulose with 12 mL of deionized water, shake well, add 120 mL of 4° C. precooled 85% phosphoric acid, stir well, and obtain a milky white cellulose phosphoric acid suspension. Incubate in a low-temperature shaking incubator at 4°C and 200rpm for 24 hours to obtain a transparent and viscous cellulose phosphate solution, add 600mL deionized water to dilute, mix well, let stand for 2 hours,...
Embodiment 3
[0035] Example 3: Regenerated cellulose-myofibrillar protein gel
[0036] Step (1), extraction of myofibrillar protein: remove pig hind leg connective tissue and fat, cut into small pieces and grind; Add 400mL standard salt solution (0.1mol / L NaCl, 20mmol / L Na 2 HPO 4 / NaH 2 PO 4 , 2mmol / L MgCl 2 , 1mmol / L EGTA, pH value is 6.5), after homogenization, centrifuge at 2000g for 15min, and repeat the homogenization and centrifugation twice; Centrifuge at 2000g for 15min to obtain myofibrillar protein;
[0037] Step (2), preparation of regenerated cellulose: Wet 3 g of microcrystalline cellulose with 12 mL of deionized water, shake well, add 120 mL of 4° C. precooled 85% phosphoric acid, stir well, and obtain a milky white cellulose phosphoric acid suspension. Cultivate in a low-temperature shaking incubator at 4°C and 200rpm for 24 hours to obtain a transparent and viscous cellulose phosphate solution and let it stand for 2 hours, then add 600mL deionized water to dilute and...
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