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Method for identifying male and female trees based on paper chromatography

A technology of paper chromatography and trees, which is applied in the direction of analyzing materials, preparation of test samples, material analysis through optical means, etc., can solve the problems of pedestrians and environmental adverse effects, inability to select male and female, low survival rate, etc., and achieve POD The effect of enhanced isoenzyme activity, low identification cost, and simple operation

Active Publication Date: 2019-05-07
TARIM UNIV
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Problems solved by technology

However, adult Populus euphratica female trees are full of fruit flocs in autumn, which is likely to cause adverse effects on pedestrians and the environment, and the cutting technology of Populus euphratica is not yet mature because of the low survival rate, so it has not been widely used, so the propagation method of Populus euphratica is still propagated by seeds Mainly, male and female cannot be selected when raising seedlings, and need to be identified later

Method used

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  • Method for identifying male and female trees based on paper chromatography
  • Method for identifying male and female trees based on paper chromatography

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Embodiment Construction

[0023] 1. Identification method

[0024] 1.1 Instrument reagents

[0025] (1) Instruments: mortar, gauze, beaker, 5mL centrifuge tube, chromatography filter paper strip (length 15cm, width 2.5cm), capillary tube, petri dish, pipette, graduated cylinder, etc.

[0026] (2) Reagents: acetic acid, benzidine, hydrogen peroxide, ethanol.

[0027] 1.2. Reagent preparation

[0028] (1) Acetic acid-benzidine dye solution: Weigh 1 g of benzidine, first dissolve it with 18 mL of warm glacial acetic acid, then add 2 mL of distilled water, and store it at room temperature after it is completely dissolved. When using, take 0.5mL mother liquor, add 9.3mL distilled water, add 0.2mL, 3wt% H 2 O 2 Solution, mix well.

[0029] (2) 20% ethanol solution: Take 20 mL of absolute ethanol into a beaker, add distilled water to make the volume up to 100 mL, and bottling for use.

[0030] 1.3. Identification method

[0031] (1) Protein extraction: when the leaves have turned yellow in autumn, remove the leaves and w...

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Abstract

The invention discloses a method for identifying male and female trees based on paper chromatography, comprising the following steps of: (1) protein extraction; (2) spotting; (3) chromatography: using20%-30% ethanol solution as anti-male chromatography solution; (4) staining; and (5) male and female identification. The method is simple, reliable in a result, low in the identification cost, and convenient for popularization and application.

Description

Technical field [0001] The invention relates to the technical field of male and female identification of trees. Specifically, it is a method for identifying male and female trees based on paper chromatography. Background technique [0002] Dioecious plants refer to a type of plant that can be divided into female trees and male trees. Female trees have only female flowers and male trees have only male flowers. Approximately 5% of plants in nature are dioecious. Because of the differences in some characteristics such as results, people often need to identify male and female at the seedling stage when using dioecious plants. In the early days, people used the subtle morphological characteristics of male and female plant seedlings to identify them. However, the types of these methods are very limited and the accuracy is also very low. Currently, the methods used for exploration include isoenzyme method and SSR method. The accuracy is high, but special instruments are required, whic...

Claims

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Application Information

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IPC IPC(8): G01N21/84G01N1/30
Inventor 赵利峰秦少伟罗生杰王汝鲜王元康王硕李永乐李娇娇
Owner TARIM UNIV
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