A method for breeding Portuguese oyster tetraploids

Abstract

The invention provides a method for culturing a Crassostrea angulata tetraploid. The method comprises the steps of firstly culturing a pacific oyster tetraploid, hybridizing the pacific oyster tetraploid with Crassostrea angulata so as to culture a hybridized triploid, fertilizing the hybridized Crassostrea angulata triploid with a Crassostrea angulata diploid sperm, carrying out anesthetization by virtue of magnesium chloride mixed liquid to inhibit the release of a second polar body so as to generate the tetraploid, wherein an inductive agent is safe and toxic-free. According to the method,the long induction process of the Crassostrea angulata triploid is solved, the survivable and stable tetraploid Crassostrea angulata can be cultured by virtue of the technical routes of hybridization,high permeation, anesthetization, molecular marking and the like, and the foundation is laid for the large-scale seedling growing of triploid Crassostrea angulata.

Description

technical field [0001] The invention belongs to the technical field of shellfish breeding, and relates to a method for cultivating polyploid Portuguese oysters, in particular to a method for cultivating tetraploid Portuguese oysters by hybridization. Background technique [0002] Oyster is the most widely cultivated and most productive marine economic shellfish in the world. Its meat is delicious and nutritious, and it is known as "milk in the sea". In 2017, my country's oyster farming output reached 4.8794 million tons, ranking first in the world, of which Portugal had the highest oyster output, accounting for more than 40% of the total output. The Portuguese oyster (Crassostrea angulata) belongs to the phylum Mollusca, class Bivalvia, order Pterioida, and family Ostreidae. It is one of the most productive oyster species in China and even in the world. [0003] However, in the long-term Portuguese oyster farming process, there are also many problems, which are mainly refle...

AI Technical Summary

Problems solved by technology

At present, there are mainly two ways to obtain triploids of Portuguese oysters. One is to directly induce triploids, but there are three problems in this method: the induction rate of triploids is difficult to reach 100%; Stress results in lower larval survival; triploid individuals are incapable of self-sustaining populations due to their sterility

The second is to induce tetraploid Portuguese oysters, and obtain triploid offspring through tetraploid and diploid crosses, but obtaining tetraploid Portuguese oysters is the technical bottleneck

[0005] At present, with regard to the cultivation of oyster tetraploids, the surviving Oyster tetraploids can be cultivated in China, mainly using the sperm of male Oyster diploids and the eggs of female Oyster triploids invented by American scholars, and fertilized by cells The inducer of relaxin B suppresses the discharge of the first polar body of the fertilized egg to induce tetraploidy, but on the one hand, the inducer is highly toxic and carcinogenic, which has a great impact on the embryo and the environment; on the other hand, this method Tetraploid offspring induced by the method are less stable, prone to chromosome loss, etc., and need to be frequently induced and cultivated