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Microcavity multicolor fluorescent digital PCR chip and application thereof

A microcavity and chip technology, applied in laboratory containers, enzymology/microbiology devices, microbial measurement/inspection, etc., can solve the problems of low system integration, expensive equipment, cumbersome operation, etc., and achieve high Detection sensitivity, easy operation, and the effect of improving reaction efficiency

Pending Publication Date: 2019-05-10
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current digital PCR system mainly has the following deficiencies: the integration of the system is low
QX200, Raindrop, and QuantStudio systems all need to use two to three different instruments to complete the steps of digital PCR sample dispersion, amplification, and detection, which are cumbersome and expensive.
During the transfer and switching of samples in these instruments, there is a risk of contamination, resulting in false positive signals

Method used

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  • Microcavity multicolor fluorescent digital PCR chip and application thereof
  • Microcavity multicolor fluorescent digital PCR chip and application thereof
  • Microcavity multicolor fluorescent digital PCR chip and application thereof

Examples

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Embodiment 1

[0030] (1) Chip structure

[0031] like figure 1 As shown, this embodiment provides a microcavity multicolor fluorescent digital PCR chip, the chip material is polydimethylsiloxane (polydimethylsiloxane, PDMS), the chip adopts double-sided liquid separation and double-sided waste liquid storage The design can greatly speed up the speed of sample injection and liquid separation, and shorten the experiment time. The chip structure includes a liquid inlet, a microcavity array area, and a waste liquid bin. The liquid inlet includes a sample inlet and an indicator inlet. The waste liquid bin includes a sample waste liquid bin and an indicator waste liquid bin. The microcavity The array area includes a microcavity reaction array group and a microcavity indicating array group; the liquid inlet communicates with each microcavity in the microchamber array area through a micropipe and is further connected with a waste liquid bin. Wherein, the sample inlet is used for entering the PCR...

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Abstract

The invention relates to a microcavity multicolor fluorescent digital PCR chip and application thereof. The chip comprises a liquid inlet port, a double side microcavity array and a double side wasteliquid tank. The chip is used for rare cancer rare mutation analysis, differential gene expression analysis or high precision copy number variation analysis. The multicolor fluorescence detection is used, so that the detection flux of a single chip can be greatly improved, the reaction efficiency is greatly improved, the operation is convenient, the speed is high, extremely high detection sensitivity can be achieved compared with an existing real-time fluorescence quantitative PCR technology, and the application prospect is good.

Description

technical field [0001] The invention belongs to the field of liquid biopsy, in particular to a microcavity multicolor fluorescent digital PCR chip and its application. Background technique [0002] In recent years, with the change of residents' lifestyle and factors such as environmental pollution, malignant tumors have become the biggest killer of residents' health. In 2012, approximately 14.1 million people were diagnosed with cancer and 8.2 million people died of cancer (equivalent to 14.6% of the total annual death toll). Due to the extremely complex etiology and pathogenesis of cancer, and individual differences in clinical manifestations, most patients have developed to an advanced stage when they are diagnosed. Clinical statistics show that, taking lung cancer as an example, its overall 5-year survival rate is only 10%-16%, while the 5-year survival rate of stage I patients can reach 65-75%. Therefore, early detection and early treatment have a positive effect on pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/00
CPCB01L3/502784B01L7/52B01L2300/0861C12Q1/6851C12Q2531/113C12Q2563/107
Inventor 毛红菊白亚楠屈友兰武振华赵建龙
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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