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Culture medium treatment method for increasing number of lactobacillus acidophilus fermentation and lyophilization viable bacteria

A technology of Lactobacillus acidophilus and fermentation medium, applied in the field of culture medium, can solve the problem of low viable count of products, and achieve the effect of increasing the viable count

Inactive Publication Date: 2019-05-14
BIOGROWING CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There have been many domestic researches on Lactobacillus acidophilus, but the number of live bacteria in the product is relatively low

Method used

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Embodiment Construction

[0011] The present invention will now be further described.

[0012] The classification name of the Lactobacillus acidophilus used in the present invention is LA-G80 (Lactobacillus acidophilus LA-G80), and the preservation place is: China Typical Culture Collection Center, the preservation number is: CCTCC NO: M2013337, and the preservation time is: July 2013 16th.

[0013] The present invention is a kind of culture medium treatment method that improves fermentation and freeze-drying viable count of Lactobacillus acidophilus, carries out according to the following steps:

[0014] Step 1: Put 200ml of MRS seed medium in a 250ml flat-bottom bottle, inoculate the preserved Lactobacillus acidophilus glycerin tube, place it in a constant temperature incubator at 37°C and let it stand for 12 hours to form Lactobacillus acidophilus seeds.

[0015] Step 2, transfer 2% Lactobacillus acidophilus seeds to 200ml MRS seed medium, and cultivate for 6 to 8 hours to form 2% Lactobacillus aci...

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Abstract

The invention relates to the technical field of strain culture mediums, in particular to a culture medium treatment method for increasing the number of lactobacillus acidophilus fermentation and lyophilization viable bacteria. Compared with the prior art, according to the nutritional requirements of the lactobacillus acidophilus, on the basis of a bacterium enrichment test by adding organic pea protein powder, impurities obtained after centrifugation, emulsification and lyophilization of fermentation liquid are reduced to improve the number of the viable bacteria of lyophilization lactobacillus acidophilus powder.

Description

technical field [0001] The invention relates to the technical field of strain culture medium, in particular to a culture medium treatment method for increasing the number of fermented and freeze-dried live bacteria of Lactobacillus acidophilus. Background technique [0002] Lactobacillus acidophilus is one of the beneficial bacteria inhabiting the human intestinal tract. When there is a certain amount, it can favorably regulate the balance between beneficial and harmful microbial flora in the intestinal tract, thereby achieving the effect of promoting the health of the host. It is currently used as In the lactic acid bacteria family, probiotics that attach great importance to research and development are regarded as the third generation of lactic acid starter strains. Live lactic acid bacteria preparations regulate the micro-ecological balance of their hosts through the active life activities of the live bacteria contained in them, and the level of live bacteria is always a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/23
Inventor 宋锦安马章献陈齐郑建丰
Owner BIOGROWING CO LTD
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