Method for rapidly breeding and preserving germplasm resources of Chinese yams

A technology for yam and germplasm, applied in the directions of vegetative reproduction, botanical equipment and methods, horticulture, etc., can solve the problems of high technical difficulty, large impact of soil-borne diseases, high production cost, and solve the problem of high cultivation cost and no pesticides. and hormone residues, the effect of improving efficiency

Inactive Publication Date: 2019-05-17
JIANGSU ACAD OF AGRI SCI
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  • Abstract
  • Description
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Problems solved by technology

[0004] The present invention provides a method for rapid propagation and preservation of yam germplasm resources in view of the problems of cumbersome operation, high technical difficulty requirements, high production cost, large influence of seasons, hormone residues and soil-borne diseases in the existing yam propagation technology. , Simplify the reproduction and preservation technology of yam germplasm resources, reduce the cost of yam reproduction and preservation, and improve the reproduction efficiency

Method used

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  • Method for rapidly breeding and preserving germplasm resources of Chinese yams
  • Method for rapidly breeding and preserving germplasm resources of Chinese yams
  • Method for rapidly breeding and preserving germplasm resources of Chinese yams

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1 Materials and methods

[0019] 1.1 Material: The material used in the test is ginseng potato variety, Suling No. 1

[0020] 2 test method

[0021] 2.1 Effects of different culture methods on the survival rate of stem cuttings

[0022] The yam varieties that grow normally in the field are divided into tender stems (1 to 3 nodes at the top, that is, 1 to 3 nodes at the top of the plant are tender stems) and mature stems, and cut into sections according to the nodes. 1 piece of leaf, cuttage in the tissue culture bottle, the specification of the tissue culture bottle is ZP17-440 (Shanghai Jiafeng Horticultural Supplies Co., Ltd.). The cutting medium is tap water. Tap water was added to the tissue culture bottle, and the stem segments were immersed in water. The water does not cover the stem nodes, and the leaves do not immerse in the water. Normal temperature, dark cultivation and constant temperature cultivation were adopted respectively. The constant temperature c...

Embodiment 2

[0043]1 Materials and methods

[0044] 1.1 Materials: The materials used in the test are divided into yams, ginseng, brown yams and yams. The variety is Fujian snow potato, and the yam varieties are Guangdong Huaishan and Guangxi Huaishan.

[0045] 1.2 Test method

[0046] For yam varieties that grow normally in the field, remove the tender stems (1 to 3 nodes at the top), cut them into sections, keep at least 1 node in each section, and have at least 1 leaf in each section. In a container with tap water, the water does not cover the stem nodes, and the leaves do not immerse in the water. It is cultured in a culture room by means of constant temperature culture. The temperature of the culture room is 25±2°C, the light intensity is 2000-3000lx, and the light is 12h / d every day. During the cultivation process, the water was changed every two weeks. During the cultivation process, the growth of cutting stem segments was observed.

[0047] The results showed that the cutting...

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Abstract

The invention discloses a method for rapidly breeding and preserving germplasm resources of Chinese yams and belongs to the technical field of agricultural production. According to the method, Chineseyam plants growing normally in fields are cut into segments according to sections and are cultured in a constant-temperature culture room in a water culture mode, and water is adopted as a cuttage medium. After 3 days of cuttage, yams can form bulbil directly, and the weight of the bulbil can be up to about 0.5g at the 30th day; dioscorea alata, dioscorea persimilis and mountainous potatoes startto germinate from the 7th day, roots grow out at the 15th day, micro tubers are formed 20 days later; after 60 days of culture, buds are cut for a second time of cuttage, and the operation is repeated till the purpose of rapidly breeding and preserving germplasm resources of the Chinese yams; seedlings growing from rooting can be directly transplanted and survive without seedling strengthening. The segments of the Chinese yams are cut in the water culture mode, rapid breeding and preservation of Chinese yam seedlings and potato seeds can be achieved, and the method has the characteristics ofbeing simple and convenient to operate, low in technique difficulty requirement, low in breeding and preservation cost and high in efficiency.

Description

technical field [0001] The invention relates to a method for rapid propagation and preservation of yam germplasm resources, belonging to the technical field of agricultural production. Background technique [0002] Yam is an important plant group of the genus Dioscorea (Dioscorea L.) in the family Dioscoreaceae, which includes many plants of the genus Dioscorea. At present, there are four kinds of Dioscorea plants, Dioscorea opposita Thunb, Dioscorea alata, Dioscorea persimilis and Dioscorea fordii, which are widely planted and produced in my country. Yam has a cultivation history of more than 2,000 years in my country. In addition to being used as medicine, it is also one of the important food crops in my country. Yam is usually propagated by tubers, which has a low reproduction coefficient and high production cost, and there are preservation problems for some difficult-to-preserve yam resources. The rapid propagation technology of yam tissue culture can increase the repr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/10A01G31/00
Inventor 韩晓勇殷剑美张培通王立郭文琦李春宏
Owner JIANGSU ACAD OF AGRI SCI
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