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a urine preservation solution

A technology for preserving liquid and urine, applied in the field of cell culture, can solve the problems of easy occurrence of bacterial and fungal contamination, and achieve the effect of prolonging the preservation time, preventing bacterial and fungal contamination, and simplifying the operation.

Active Publication Date: 2022-05-24
FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems in the prior art that cells must be separated immediately after urine collection and are prone to bacterial and fungal contamination, the purpose of the present invention is to provide a urine preservation method that can obtain epithelial cells from urine in a practical manner. liquid, as well as a urine collection container and a method and kit for collecting and preserving urine, so as to preserve the epithelial cells in the urine for a long time and provide sufficient operation time for actually obtaining the epithelial cells

Method used

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Embodiment approach

[0049] According to a preferred embodiment of the present invention, the rotating device may comprise:

[0050] A rotating handle is arranged at the bottom of the urine collection container;

[0051] a transmission rotary rod, which is integrally formed of plastic together with the rotary handle, and is provided with a sealing thread at one end connected with the rotary handle; and

[0052] a rubber stopper, which has a circular sheet-like structure and is arranged at the opposite end of the transmission rotary rod and the rotary handle;

[0053] The bottle body is provided with a circular hole on the bottom surface, the sealing thread and the circular hole on the bottom surface are threadedly engaged to seal the bottom surface, and the diameter of the hole of the intermediate partition plate is larger than the diameter of the transmission rod. The diameter is smaller than the diameter of the rubber stopper, the transmission screw rod passes through the storage liquid sump so...

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Abstract

The invention provides a urine preservation solution for obtaining epithelial cells from urine, which contains an acid-base balance buffer system, various ionic components, various amino acid components and inhibitors of bacteria or fungi, and does not contain animal source ingredients. The present invention also provides a urine collection container, which includes: a bottle body, including a middle partition to divide the bottle body into an upper sample chamber and a lower preservation liquid chamber, the preservation liquid chamber is pre-filled with urine preservation liquid; The bottle cap is arranged on the top of the urine collection container; and the rotating device is configured to close or open the hole in the middle partition to close or open the communication between the sample chamber and the preservation liquid chamber. The invention also provides a method for collecting and preserving urine for culturing epithelial cells, and a corresponding kit. In this way, the collection of urine samples is simplified, the storage time of epithelial cells in urine is prolonged, and it is convenient to obtain epithelial cells from urine.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a urine preservation solution, a urine collection container, a method and a kit for collecting and preserving urine, which can actually be used to obtain epithelial cells from urine. Background technique [0002] In recent years, pluripotent induced stem cell technology is an important breakthrough in the field of stem cell research. This technology uses autologous cells to be prepared by reprogramming technology, which not only avoids the ethical problems of stem cell application, but also solves the problem of immune rejection in cell therapy. future treatments for diseases. Collecting and extracting patient sample cells as reprogrammed cells is the initial work for the preparation of pluripotent induced stem cells. At present, skin fibroblasts isolated from patient skin tissue and mononuclear cells isolated from peripheral blood are mostly used. However, the above cell c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02C12N5/071G01N1/38G01N1/10
Inventor 刘尚雨郑黎晖姚焰
Owner FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCI & PEKING UNION MEDICAL COLLEGE
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