Nanoparticles for Raman spectrum detection of oral squamous cell carcinoma tumor cells and preparation method thereof

Abstract

The invention provides nanoparticles for Raman spectrum detection of oral squamous cell carcinoma tumor cells. The nanoparticles for Raman spectrum detection of the oral squamous cell carcinoma tumorcells are prepared from nanoscale gold particles and 4-mercaptobenzoic acid, alpha-sulfydryl-omega-carboxyl polyethylene glycol and an EGFR antibody through response. The nanoparticles for Raman spectrum detection of oral squamous cell carcinoma tumor cells can be combined with the characteristics of the oral squamous cell carcinoma tumor cells, so that specific Raman signals are obtained, and theoral squamous cell carcinoma tumor cells are rapidly and accurately detected in real time in situ; compared with traditional frozen pathological tissue section examination, the time is saved, accuracy is improved, and a novel detection method for detecting whether oral squamous cell carcinoma cells remain or not in clinical operations is provided.

Description

technical field [0001] The invention relates to the medical field, in particular to a nanoparticle used for Raman spectrum detection of oral squamous cell carcinoma tumor cells and a preparation method thereof. Background technique [0002] According to statistics from the National Cancer Research Agency of the World Health Organization, there are about 300,000 new oral cancer patients and about 120,000 deaths each year worldwide. Compared with the 1960s, the incidence of oral cancer has increased by 15%, becoming the sixth most common malignant tumor in the whole body. In the United States, more than 100 patients are diagnosed with oral cancer every day, and its mortality rate is much higher than that of general cancers. The 5-year survival rate is only 50% to 60%, and almost one patient dies of oral cancer every hour. [0003] Epidermal growth factor receptor (EGFR) belongs to the ErbB receptor family and is a tyrosine kinase-type transmembrane receptor that regulates the...

AI Technical Summary

Problems solved by technology

The nanoparticles commonly used for SERS detection are mostly unmodified bare nanoscale gold or silver particles. These nanoparticles need to be applied in complex biological tissue environments without surface modification. Non-specific binding of macromolecules, unable to specifically bind to EGFR protein

This naked nanoprobe without surface modification is easy to bind to some impurity molecules and cannot specifically bind to the detection target molecule. At the same time, the naked probe is prone to agglomeration and causes false positive signals.

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