Molecular marking method and application analysis of soybean grain protein related wild fragments
A molecular marker and protein technology, applied in the fields of application, botanical equipment and methods, plant genetic improvement, etc., can solve problems such as being easily affected by the environment, and achieve the effect of improving efficiency, improving yield and quality, and shortening breeding cycle.
Inactive Publication Date: 2019-05-24
JIANGSU COASTAL AREA AGRI SCI RES INST
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Problems solved by technology
The phenotype of soybean grain protein is easily affected by the environment, and there are few selective combinations of parental markers for soybean breeding, so the detection of soybean grain protein-related QTL is of great significance for the development of soybean molecular marker-assisted breeding
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[0016] In this study, wild soybean N24852 and cultivated soybean NN1138-2 with significant differences in grain traits were selected as parents, and a set of SojaCSSLP consisting of 195 high-generation families was constructed. 4 The group is the material. In this experiment, cultivated soybean NN1138-2 (MG V) was selected as the female parent. It is the main cultivar in the middle and lower reaches of the Yangtze River. The plant height is about 80cm, the hundred-grain weight is 15.2-22.3g, the grain protein is 42%-46%, the grain oil content is 18%-22%, and the lodging resistance is strong. Wild soybean N24852 (MG III) is the male parent, matures later than NN1138-2, the pods and seed coats are black, with mud film, the stems are entangled, sprawling, the weight of 100 seeds is about 2g, and the plant height is more than 150cm. The protein is 43% to 48%, and the oil content of the seeds is about 9% to 12%. Differences between the two parents in some qualitative and quantita...
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The invention relates to a molecular marking method of soybean grain protein related wild fragments. The molecular marking method includes the steps: 1) soybean chromosome fragment substitution line population grain protein distribution: continuously planting NN1138-2 and N24852 advanced line SojaCSSLP4 populations in two environments, and counting grain protein content; 2) soybean grain protein wild fragment positioning: building a genetic map by an SSR (simple sequence repeats) technology to position grain protein fragments of the soybean SojaCSSLP4 populations and acquire 9 grain protein related wild fragment sites. A marking combination is used for low-generation selection of breeding materials, materials with high grain protein content can be gathered in the low-generation materials,breeding progress is accelerated, and breeding scale is reduced.
Description
technical field [0001] The invention relates to the technical field of soybean molecular marker assisted breeding, in particular to the application analysis of molecular markers and marker combination assisted breeding of soybean protein-related wild fragments. Background technique [0002] Soybean is one of the important crops in our country. It originated in China. The protein content in its grain is about 40%, which is the main source of human plant protein. Soybean mainly includes annual wild soybean (G.soja Sieb.&Zucc.) and cultivated soybean (G.max(L)Merr.), which is the primary gene bank for soybean breeding. The grain protein of wild soybean varies from 39.2% to 54.2%, and that of cultivated soybean varies from 38.9% to 45.3%. There are excellent germplasm resources with high grain protein in wild soybean, including allelic variation sites controlling high grain protein. [0003] Soybean grain protein is an important quality trait and a complex quantitative trait c...
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IPC IPC(8): C12Q1/6895A01H1/02A01H1/04
Inventor 杨红燕沈会权张英虎
Owner JIANGSU COASTAL AREA AGRI SCI RES INST