A potentiometric pH sensor based on a tryptophan polypeptide oxide film and its preparation method and application
An oxide film, ph sensor technology, applied in the field of sensors, to achieve the effect of high stability and strong anti-stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] Example 1: Preparation of a potentiometric pH sensor based on tryptophan polypeptide oxide membrane
[0028] The polypeptide used in this example was synthesized by Shanghai Qiangyao Biotechnology Co., Ltd., and its amino acid sequence is Lys-Phe-Trp-Gly-Lys.
[0029] The potentiometric pH sensor based on the tryptophan polypeptide oxide film of this embodiment is prepared by the following method:
[0030] (1) Use 0.05mol / L phosphate buffer solution as the electrolyte, take the polypeptide as the film-forming reactant, and prepare a polypeptide solution with a concentration of 0.001mol / L as the electropolymerization solution; the temperature of the polypeptide solution is 25±10°C;
[0031] (2) Immerse the graphite electrode in the polypeptide solution of step (1) as the working electrode, use the titanium sheet as the counter electrode, and the saturated calomel electrode as the reference electrode, connect with the electrochemical workstation, and set the potential ran...
Embodiment 2
[0032] Example 2: Establishment of a method for detecting the pH value of a solution with a potentiometric pH sensor based on a tryptophan polypeptide oxide film
[0033] The method for detecting the pH value of the solution based on the potentiometric pH sensor of the tryptophan polypeptide oxide film to illustrate the establishment of the embodiment 1 is obtained by the following method:
[0034] The potentiometric pH sensor based on the tryptophan polypeptide oxide film prepared in Example 1 was used as an indicator electrode, and the conditional potential and the open circuit potential were measured linearly with pH;
[0035] The result is as figure 1 As shown, the conditional potential of the tryptophan polypeptide oxide membrane shifts negatively with the increase of pH. In the range of pH 1 to 10, there is a good linear relationship between the conditional potential and pH, and the slope of 60.8mV / pH is close to the theoretical value. pH response slope; more importantl...
Embodiment 3
[0036] Example 3: Practical application of potentiometric pH sensor based on tryptophan polypeptide oxide film
[0037] For the actual application of the method for detecting the pH value of the solution based on the potentiometric pH sensor of the tryptophan polypeptide oxide film in Example 2, the specific operation steps are as follows:
[0038] Using the potentiometric pH sensor based on the tryptophan polypeptide oxide film prepared in Example 1 as an indicator electrode, measure the pH of deionized water, milk and milk water samples according to the conditional potential and open circuit potential versus pH linear change curve value.
[0039] The result is as image 3 As shown, the measured pH values of the potentiometric pH sensor based on the tryptophan polypeptide oxide film and the commercial glass pH sensor are close to each other for the determination of deionized water; The pH value measured by the membrane potentiometric pH sensor does not change significantl...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More