A method for extracting and purifying vitamin K2 from wall-broken Bacillus natto cells

A technology of bacillus natto and vitamins, applied in the field of bioengineering and bioseparation engineering, can solve the problems of complex overall process, low extraction rate, high cost, etc., and achieve the effect of simple process, high efficiency and high extraction rate
CN109824496BActive Publication Date: 2021-07-27HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
Publication Date
2021-07-27

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Abstract

A method for extracting and purifying vitamin K2 from broken Bacillus natto cells, separating the fermented microorganisms from solid and liquid by ultrafiltration and centrifugation to obtain wet cells, and treating the wet cells with ion beams to break the walls , Extracted with absolute ethanol, not only will obtain a large amount of vitamin K2, but also extract less oil, rough purification is carried out during the extraction process, which provides a good solution for the subsequent one-step high-efficiency purification. The extract is extracted through a column, and organic reagents with different polarities are used for extraction and purification, and impurities of different polarities are extracted, so that the purity of the solution is greatly improved. It is then frozen and crystallized to obtain vitamin K2 crystals with a purity of 95%. The method omits the processes of drying and cell crushing in the traditional method, and improves the extraction efficiency and the extraction purity. And through one-step purification to obtain vitamin K with higher purity 2 .
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Description

technical field

[0001] The invention relates to a method for extracting and purifying vitamin K2 from broken Bacillus natto thallus, belonging to the fields of bioengineering and bioseparation engineering. Background technique

[0002] Vitamin K 2 It is a general term for a series of terpene side chain compounds containing 2-methyl-1,4-naphthoquinone core and C3 with different numbers of isoprene structural units. According to the carbon element on the terpene side chain The number can be divided into K2(10), K2(20), K2(35), K2(40) and so on. Vitamin K 2 It is a fat-soluble vitamin, a derivative of a naphthoquinone group with biological activity of phylloquinone, and is one of the indispensable important vitamins in the human body. With research on its function, it has been proven that vitamin K 2 It has the effect of preventing osteoporosis, cardiovascular, Parkinson, liver cancer and other diseases. Therefore, functional foods, medical products, cosmetics and other fi...

Claims

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