Bacillus amyloliquefaciens Y1711, and culture method and application thereof

A technology of amylolytic bacillus and bacillus, applied in the field of microorganisms, can solve the problems of inability to promote the growth of beneficial green algae, and achieve the effects of improving the aquaculture environment, increasing the growth rate, and reducing nitrite

Active Publication Date: 2019-06-04
INST OF AQUATIC LIFE ACAD SINICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the Bacillus amyloliquefaciens disclosed in patent CN106479921A can degrade ammonia...

Method used

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  • Bacillus amyloliquefaciens Y1711, and culture method and application thereof
  • Bacillus amyloliquefaciens Y1711, and culture method and application thereof
  • Bacillus amyloliquefaciens Y1711, and culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation and screening of Bacillus amyloliquefaciens Y1711

[0021] Sampling: Select 3 different sampling points in the culture pond with high ammonia nitrogen to take the sediment, put it into a sterile ziplock bag, seal it, and bring it back.

[0022] Separation: Dilute the bottom sludge with sterile water at a ratio of 1g:10mL, treat it in a water bath at 80°C for 10 minutes, draw 200μl of the bacterial solution and spread it on a starch medium plate, and incubate at 37°C.

[0023] Purification: After 24 hours of plate culture, pick the strains that produce hydrolysis circles and streak them on the nutrient agar medium, then pick single bacteria with irregular colony edges, dry surface and wrinkled surfaces, and continue to streak and separate until pure cultures are obtained.

[0024] Screening: inoculate the purified bacterial strains on the starch medium plate again, and measure the colony diameter (C) and the hydrolysis circle diameter (H). The strain...

Embodiment 2

[0025] Example 2 Identification of Bacillus amyloliquefaciens Y1711

[0026] 1. Morphological identification and detection of physiological and biochemical characteristics

[0027] Bacillus amyloliquefaciens Y1711 was inoculated on a nutrient agar plate, cultured at 37°C for 24 hours, and the growth of the colony was observed. The bacteria were picked for Gram staining, and the morphology and spore formation of the bacteria were observed. Physiological and biochemical characteristics were tested according to the Berger Manual of Bacteria.

[0028] On the nutrient agar plate, the strains have irregular edges, dry and wrinkled surface; Gram-positive, rod-shaped bacteria, spores, and enlarged cysts. The strain's nitrate test, contact enzyme test, starch hydrolysis, and D-mannitol acid production test were all positive.

[0029] 2. Molecular biological identification

[0030] A single colony of Bacillus amyloliquefaciens Y1711 was picked and inoculated in nutrient broth culture...

Embodiment 3

[0035] The laboratory water purification function measurement of embodiment 3 bacillus amyloliquefaciens Y1711

[0036] Prepare simulated aquaculture water indoors. The simulated aquaculture water is prepared from shrimp bait soaking solution, ammonium nitrate, dipotassium hydrogen phosphate, and sodium nitrite, and the salinity is adjusted to 15 with sea crystals, pH7.8±0.5. The total content of ammonia nitrogen in the simulated aquaculture water was 7.79 mg / L, and the total content of nitrite was 1.03 mg / L.

[0037] Add Bacillus amyloliquefaciens Y1711 bacterium liquid to simulated culture water, make the bacteria content wherein be 10 6 cfu / mu, before adding the bacteria solution and on the 1st, 2nd, 3rd, 5th, and 7th days after adding the bacteria solution, water was taken to measure the content of ammonia nitrogen and nitrite in the water. The data are shown in Table 1 below.

[0038] The culture method of Bacillus amyloliquefaciens Y1711 bacteria liquid is as follows: n...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus amyloliquefaciens Y1711, and a culture method and application thereof. The bacillus amyloliquefaciens Y1711 is Bacillus amyloliquefaciens Y1711, the preservation number of the bacillus amyloliquefaciens Y1711 in the China Center for Type Culture Collection is CCTCC NO:M 2018356, and the preservation date is June, 11, 2018. The bacillus amyloliquefaciens Y1711 disclosed by the invention can enable ammonia nitrogen and nitrite in breeding water bodies to be obviously reduced and enable the growthrate of beneficial green alga to be obviously increased, so that a benign circulation is formed, and besides, the bacillus amyloliquefaciens Y1711 is harmless to men and cattle, and can be in large-scale production for improving aquaculture environment.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus amyloliquefaciens Y1711, a cultivation method and an application thereof. Background technique [0002] With the continuous increase of aquaculture area in my country, the discharge of aquaculture wastewater and the accumulation of organic silt formed by residual bait, feces, and biological debris have increased the organic pollution of aquaculture water, and the quality of the aquaculture ecological environment has deteriorated year by year. The water quality of some aquaculture water bodies is seriously aging, and the aquaculture parameters such as ammonia nitrogen and nitrite in the water body continue to deteriorate; the degree of beneficial algae activity is greatly reduced, and the situation of aging and stagnant water is becoming more and more serious. Bacillus is a common probiotic used to repair the breeding environment by using microorganis...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38C12N1/12C02F3/34C12R1/07C02F101/16
CPCY02W10/10
Inventor 王启烁吴晓娇张善夫张景龙孙家凯
Owner INST OF AQUATIC LIFE ACAD SINICA
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