Bacillus amyloliquefaciens Y1711, and culture method and application thereof
A technology of amylolytic bacillus and bacillus, applied in the field of microorganisms, can solve the problems of inability to promote the growth of beneficial green algae, and achieve the effects of improving the aquaculture environment, increasing the growth rate, and reducing nitrite
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Embodiment 1
[0020] Example 1 Isolation and screening of Bacillus amyloliquefaciens Y1711
[0021] Sampling: Select 3 different sampling points in the culture pond with high ammonia nitrogen to take the sediment, put it into a sterile ziplock bag, seal it, and bring it back.
[0022] Separation: Dilute the bottom sludge with sterile water at a ratio of 1g:10mL, treat it in a water bath at 80°C for 10 minutes, draw 200μl of the bacterial solution and spread it on a starch medium plate, and incubate at 37°C.
[0023] Purification: After 24 hours of plate culture, pick the strains that produce hydrolysis circles and streak them on the nutrient agar medium, then pick single bacteria with irregular colony edges, dry surface and wrinkled surfaces, and continue to streak and separate until pure cultures are obtained.
[0024] Screening: inoculate the purified bacterial strains on the starch medium plate again, and measure the colony diameter (C) and the hydrolysis circle diameter (H). The strain...
Embodiment 2
[0025] Example 2 Identification of Bacillus amyloliquefaciens Y1711
[0026] 1. Morphological identification and detection of physiological and biochemical characteristics
[0027] Bacillus amyloliquefaciens Y1711 was inoculated on a nutrient agar plate, cultured at 37°C for 24 hours, and the growth of the colony was observed. The bacteria were picked for Gram staining, and the morphology and spore formation of the bacteria were observed. Physiological and biochemical characteristics were tested according to the Berger Manual of Bacteria.
[0028] On the nutrient agar plate, the strains have irregular edges, dry and wrinkled surface; Gram-positive, rod-shaped bacteria, spores, and enlarged cysts. The strain's nitrate test, contact enzyme test, starch hydrolysis, and D-mannitol acid production test were all positive.
[0029] 2. Molecular biological identification
[0030] A single colony of Bacillus amyloliquefaciens Y1711 was picked and inoculated in nutrient broth culture...
Embodiment 3
[0035] The laboratory water purification function measurement of embodiment 3 bacillus amyloliquefaciens Y1711
[0036] Prepare simulated aquaculture water indoors. The simulated aquaculture water is prepared from shrimp bait soaking solution, ammonium nitrate, dipotassium hydrogen phosphate, and sodium nitrite, and the salinity is adjusted to 15 with sea crystals, pH7.8±0.5. The total content of ammonia nitrogen in the simulated aquaculture water was 7.79 mg / L, and the total content of nitrite was 1.03 mg / L.
[0037] Add Bacillus amyloliquefaciens Y1711 bacterium liquid to simulated culture water, make the bacteria content wherein be 10 6 cfu / mu, before adding the bacteria solution and on the 1st, 2nd, 3rd, 5th, and 7th days after adding the bacteria solution, water was taken to measure the content of ammonia nitrogen and nitrite in the water. The data are shown in Table 1 below.
[0038] The culture method of Bacillus amyloliquefaciens Y1711 bacteria liquid is as follows: n...
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