GhAGD13 gene relevant with resistance of verticillium wilt and application of GhAGD13 gene

A Verticillium wilt and resistance technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of agricultural production loss, loss of resistance, lack of genetic resources for upland cotton resistance to Verticillium wilt, etc.

Inactive Publication Date: 2019-06-04
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Upland cotton lacks genetic resources for Verticillium wilt resistance, and the main disease-resistant gene has not been cloned in cotton, and the resistance mediated by the disease-resistant gene is only effective for a specific pathogen race and is easily lost due to the mutation of the pathogen The effect of resistance leads to the re-emergence and prevalence of diseases, causing huge losses to agricultural production

Method used

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  • GhAGD13 gene relevant with resistance of verticillium wilt and application of GhAGD13 gene
  • GhAGD13 gene relevant with resistance of verticillium wilt and application of GhAGD13 gene
  • GhAGD13 gene relevant with resistance of verticillium wilt and application of GhAGD13 gene

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Embodiment 1

[0048] Example 1. Acquisition and functional verification of GhAGD13 gene in upland cotton

[0049] 1. Acquisition of GhAGD13 gene in upland cotton

[0050] 1. Planting of cotton materials:

[0051] Cotton (the cotton variety is Zhongzhi Cotton KV3 (source see above), Gossypium hirsutum L.)) seeds are delinted with sulfuric acid. Select plump cotton seeds, soak them in 70% alcohol for 5 minutes, and then use 5% H 2 o 2 Soak for 2 hours, rinse with sterile water for 3 times, then soak in sterile water for 5 hours to accelerate germination. The seeds are planted in flower pots with a diameter of 11 cm (the ratio of nutrient soil to vermiculite is 2:1), placed in a greenhouse for cultivation, and the cultivation conditions are set at a temperature of 26°C, 16 hours of light, 8 hours of darkness, and a relative humidity of 70%. .

[0052] 2. Total RNA extraction:

[0053] Fresh leaves were collected to extract total cotton RNA. For RNA extraction, refer to the instructions ...

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Abstract

The invention discloses a GhAGD13 gene relevant with resistance of verticillium wilt and an application of the GhAGD13 gene, and provides an application of DNA molecules in following (1) to (3) to strengthening or reducing resistance of plants to verticillium wilt: (1) a DNA molecule of which the nucleotide sequence is as shown in SEQID No.1, (2) a DNA molecule of which the nucleotide sequence isas shown in SEQID No.3, and (3) a DNA molecule of which the nucleotide sequence is as shown in SEQID No.4. Researching the effect of the GhAGD13 gene of cotton in resistance of verticillium wilt can establish foundation for providing objective genes on culture of disease-resistant varieties by a molecular breeding method.

Description

technical field [0001] The invention relates to the field of biology, in particular to a GhAGD13 gene related to Verticillium wilt resistance and its application. Background technique [0002] Cotton is an important economic crop in the world and the main source of natural fiber. It is affected by many factors in production, among which cotton Verticillium dahliae Kleb. caused by Verticillium dahliae Kleb. The leading disease of cotton is known as the "cancer" of cotton. [0003] Verticillium dahliae is a soil-borne plant pathogen, and its dormant microsclerotia can survive in the soil for more than 10 years. It is difficult to control, and there is no ideal chemical control agent so far. At present, the most economical and effective way to control cotton Verticillium wilt in production is to breed disease-resistant varieties. [0004] Upland cotton lacks genetic resources for Verticillium wilt resistance, and the main disease-resistant gene has not been cloned in cotton, ...

Claims

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Application Information

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IPC IPC(8): C12N15/82C12N15/29A01H5/00A01H6/20A01H6/60
Inventor 张文蔚任玉红简桂良
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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