Method for removing heavy metal chromium by using dissimilatory iron-reducing bacteria to prepare bio-magnetite
A technology for dissimilating iron and magnetite is applied in the field of preparing biological magnetite to remove heavy metal Cr, which can solve the problems of limiting large-scale application process, strict microbial culture conditions, etc., and achieves overcoming toxicity inhibition, increasing treatment cost, and large reaction area. Effect
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Embodiment 1
[0041] Embodiment 1: the physical characteristics of biological magnetite
[0042] (1) The dissimilatory iron-reducing bacterium Klebsiella sp.KB52 was inoculated in a 150mL anaerobic bottle (containing 100mL basal medium) according to the inoculum amount of 1%, filled with nitrogen for 5 minutes to ensure anaerobic culture environment, 30°C, 120r / min, shaker culture ;
[0043] Basal medium composition (g / L): glucose 10, yeast extract 2.0, tryptone 1.0, NaCl 30.0, K 2 HPO 4 1.5, NaHCO 3 1.5, Fe(OH) 3 400mg / L, pH 7.0±0.2; NaHCO 3 First prepare a solution with a concentration of 50g / L, sterilize it by filtration, and add it to the basal medium at 30mL / L;
[0044] In the above-mentioned artificial synthesis of Fe(OH) 3In the redox culture system in which glucose is the electron acceptor and glucose is the electron donor, the strain KB52 can use the insoluble Fe(III) form for dissimilatory iron reduction, and the accumulated Fe(II) concentration after 96 hours of culture i...
Embodiment 2
[0047] The impact of embodiment 2 cultivation time on Cr(VI) removal rate
[0048] (1) The dissimilatory iron-reducing bacterium Klebsiella sp.KB52 was inoculated in a 150mL anaerobic bottle (containing 100mL basal medium) according to the inoculum amount of 1%, filled with nitrogen for 5 minutes to ensure anaerobic culture environment, 30°C, 120r / min, shaker culture ;
[0049] Basal medium composition (g / L): glucose 10, yeast extract 2.0, tryptone 1.0, NaCl 30.0, K 2 HPO 4 1.5, NaHCO 3 1.5, Fe(OH) 3 400mg / L, pH 7.0±0.2; NaHCO 3 First prepare a solution with a concentration of 50g / L, sterilize it by filtration, and add it to the basal medium at 30mL / L;
[0050] During the cultivation process, adopt the same method as in Example 1 to collect the biomagnetite precipitate formed in the reduction process of bacterial strain KB52 dissimilated iron every 24h, dry it into powder, and add biomagnetite to the containing In the liquid to be treated of 40mg / L Cr (VI), adjust pH ...
Embodiment 3
[0052] The influence of embodiment 3 pH value on Cr(VI) removal rate
[0053] According to the method of Example 1, when the bacterial strain KB52 was cultured in the redox culture system for 4 days, it was centrifuged, precipitated and dried to prepare bio-magnetite. Bio-magnetite was added to the liquid to be treated with different initial pH values containing 40mg / L Cr(VI) in a ratio of 2g / L, and treated for 10h, the results were as follows image 3 .
[0054] Depend on image 3 It can be seen that the bio-magnetite prepared by strain KB52 has different removal efficiencies for Cr(VI) in solutions with different pH values. When the pH value is less than 6.0, the removal efficiency of Cr(VI) by bio-magnetite is relatively high, and the removal effect is obvious. When the pH value of Cr(VI) solution was 2.0, 3.0, 4.0 and 5.0, the removal rate of Cr(VI) by biological magnetite reached 100%; when the pH value of Cr(VI) solution was 6.0, the removal rate of Cr(VI) by biolog...
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