Method for removing heavy metal chromium by preparing biological magnetite with dissimilatory iron reducing bacteria

A technology for dissimilating iron and magnetite, which is applied in the field of preparing biological magnetite to remove heavy metal Cr, can solve the problems of restricting large-scale application process and strict microbial culture conditions, and achieves the advantages of overcoming toxicity inhibition, increasing treatment cost and high concentration. Effect

Active Publication Date: 2019-06-07
TIANJIN UNIV OF SCI & TECH
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the culture conditions of microorganisms are relatively strict, which limits their large-scale application in the field of heavy metal removal.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for removing heavy metal chromium by preparing biological magnetite with dissimilatory iron reducing bacteria
  • Method for removing heavy metal chromium by preparing biological magnetite with dissimilatory iron reducing bacteria
  • Method for removing heavy metal chromium by preparing biological magnetite with dissimilatory iron reducing bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the physical characteristics of biological magnetite

[0042] (1) The dissimilatory iron-reducing bacterium Klebsiella sp.KB52 was inoculated in a 150mL anaerobic bottle (containing 100mL basal medium) according to the inoculum amount of 1%, filled with nitrogen for 5 minutes to ensure anaerobic culture environment, 30°C, 120r / min, shaker culture ;

[0043] Basal medium composition (g / L): glucose 10, yeast extract 2.0, tryptone 1.0, NaCl 30.0, K 2 HPO 4 1.5, NaHCO 3 1.5, Fe(OH) 3 400mg / L, pH 7.0±0.2; NaHCO 3 First prepare a solution with a concentration of 50g / L, sterilize it by filtration, and add it to the basal medium at 30mL / L;

[0044] In the above-mentioned artificial synthesis of Fe(OH) 3In the redox culture system in which glucose is the electron acceptor and glucose is the electron donor, the strain KB52 can use the insoluble Fe(III) form for dissimilatory iron reduction, and the accumulated Fe(II) concentration after 96 hours of culture i...

Embodiment 2

[0047] The impact of embodiment 2 cultivation time on Cr(VI) removal rate

[0048] (1) The dissimilatory iron-reducing bacterium Klebsiella sp.KB52 was inoculated in a 150mL anaerobic bottle (containing 100mL basal medium) according to the inoculum amount of 1%, filled with nitrogen for 5 minutes to ensure anaerobic culture environment, 30°C, 120r / min, shaker culture ;

[0049] Basal medium composition (g / L): glucose 10, yeast extract 2.0, tryptone 1.0, NaCl 30.0, K 2 HPO 4 1.5, NaHCO 3 1.5, Fe(OH) 3 400mg / L, pH 7.0±0.2; NaHCO 3 First prepare a solution with a concentration of 50g / L, sterilize it by filtration, and add it to the basal medium at 30mL / L;

[0050] During the cultivation process, adopt the same method as in Example 1 to collect the biomagnetite precipitate formed in the reduction process of bacterial strain KB52 dissimilated iron every 24h, dry it into powder, and add biomagnetite to the containing In the liquid to be treated of 40mg / L Cr (VI), adjust pH ...

Embodiment 3

[0052] The influence of embodiment 3 pH value on Cr(VI) removal rate

[0053] According to the method of Example 1, when the bacterial strain KB52 was cultured in the redox culture system for 4 days, it was centrifuged, precipitated and dried to prepare bio-magnetite. Bio-magnetite was added to the liquid to be treated with different initial pH values ​​containing 40mg / L Cr(VI) in a ratio of 2g / L, and treated for 10h, the results were as follows image 3 .

[0054] Depend on image 3 It can be seen that the bio-magnetite prepared by strain KB52 has different removal efficiencies for Cr(VI) in solutions with different pH values. When the pH value is less than 6.0, the removal efficiency of Cr(VI) by bio-magnetite is relatively high, and the removal effect is obvious. When the pH value of Cr(VI) solution was 2.0, 3.0, 4.0 and 5.0, the removal rate of Cr(VI) by biological magnetite reached 100%; when the pH value of Cr(VI) solution was 6.0, the removal rate of Cr(VI) by biolog...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of environmental microorganisms, and particularly relates to a method for removing heavy metal Cr (VI) by preparing biological magnetite with dissimilatory iron reducing bacteria. The dissimilatory iron reducing bacteria are used for preparing the magnetite, so that the influence of the change of culture conditions on the heavy metal removal efficiency can be reduced, the toxicity of the heavy metal to the strain can be prevented, and the removal efficiency of the heavy metal Cr (VI) is improved. When the heavy metal Cr (VI) is removed under the acidic condition, the removal rate of the heavy metal Cr (VI) can be effectively increased by 100%. The biomagnetite overcomes the toxic inhibition of heavy metals on the cell growth of the strain, and has the advantage of high Cr (VI) treatment concentration.

Description

Technical field: [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a method for removing heavy metal Cr(VI) by using dissimilatory iron-reducing bacteria to prepare biological magnetite. Background technique: [0002] Chromium pollution is the second type of heavy metal pollution after lead pollution. Heavy metal chromium usually has two valence states Cr(VI) and Cr(III), among which Cr(VI) is easily soluble in water and has strong oxidizing property, and its toxicity is 100 times that of Cr(III) (Gao Xiaopeng, Zhang Shaoqian, Xu Ping, et al. The Research Progress of Microbial Reduction of Cr(VI)[J]. Microbiology Bulletin, 2008,35(5):820-824.). Reducing Cr(VI) to Cr(III) is a key technical means to solve heavy metal Cr(VI) pollution. [0003] Compared with chemical and physical methods for chromium removal, the direct or indirect reduction of Cr(VI) by microorganisms has the advantages of in-situ treatment, low cost and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C12R1/22C02F101/22
Inventor 刘洪艳王珊
Owner TIANJIN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap