SSR molecular markers for identifying Paeonia sect. Moutan variety and application thereof

A peony and molecular marker technology, which is applied to the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problems of being easily affected by the environment, has not yet been reported, and the identification efficiency is low, and achieves reliable and accurate results. , good sensitivity, obvious difference effect

Active Publication Date: 2019-06-11
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This phenotypic identification method is generally only suitable for varieties with large phenotypic differences, and is highly susceptible to environmental influences
At present, the identification of morphological characteristics is the most intuitive method to distinguish purple spot peony varieties, but this method can only be carried out during the flowering period, and often misjudgments are caused due to subjective reasons, and the identification efficiency is low
Moreover, the system of molecular markers for the identification of peony cultivars has yet to be perfected, and the construction of a molecular ID card for cultivars has not been reported. Therefore, the identification of varieties with similar flower colors and patterns can be identified by using the unique molecular ID card of peony cultivars established by molecular markers. is very necessary

Method used

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  • SSR molecular markers for identifying Paeonia sect. Moutan variety and application thereof
  • SSR molecular markers for identifying Paeonia sect. Moutan variety and application thereof
  • SSR molecular markers for identifying Paeonia sect. Moutan variety and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Screening of SSR molecular markers and primer combinations

[0047] First, the present invention selects all 72 pairs of EST-SSRs from the five linkage groups of the first high-density genetic map of peony (Cai, 2015; Wu Jing, 2016), and synthesizes corresponding primers. The genomic DNA of Paeonia japonica was used as template DNA to amplify with these 72 pairs of EST-SSR primers, and the amplified product was detected by 6% denatured polyacrylamide gel electrophoresis combined with silver staining method, and finally screened out polymorphism rich The 34 EST-SSRs, 34 EST-SSRs and their primer information are shown in Table 2.

[0048] Table 2 34 EST-SSR molecular markers and corresponding primer information

[0049]

[0050]

[0051]

[0052] F = forward primer; R = reverse primer.

[0053] Taking the 264 varieties of P. peony in the prior art as test materials, further screening among the above-mentioned 34 EST-SSRs can realize the minimum number...

Embodiment 2

[0073] Example 2 Using the optimal combination of SSR molecular markers to establish the molecular identity cards of 264 varieties of purple spot peony

[0074] This example provides the optimal combination of 12 SSR molecular markers and their corresponding primer combinations screened in Example 1 to establish molecular ID cards for 264 varieties of Paeonia purpurea.

[0075] In the 12 pairs of primers PS095, PS356, PS166, PS119, PS271, PS068, PS367, PS074, PS157, PS221, PS159, and PS187 obtained in Example 1, all the band patterns of each pair of primers in 264 Paeonia purpurea varieties are divided by Arranged in order from small to large (the size of the amplified fragments of the two alleles is equal and the smallest is the minimum value, the size of one allele fragment remains the same, and the size of the other allele fragment increases in turn, followed by , and then rearrange the fragments that were determined to be constant allele fragments with increased sizes, and...

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Abstract

The invention relates to the technical field of plant molecular marker assisted breeding, and in particular to SSR molecular markers for identifying a Paeonia sect. Moutan variety and application thereof. The invention provides an SSR molecular marker set comprising 12 EST-SSR molecular markers and a corresponding primer set as shown in SEQ ID NO.1-24 for identifying the Paeonia sect. Moutan variety. On this basis, molecular identity cards of 264 Paeonia sect. Moutan varieties and a method for identifying the paeonia rockii varieties are established; the method has the advantages of high resolution, good sensitivity, reliable and accurate result, high efficiency, quickness and the like; according to the method, 264 different paeonia rockii varieties can be accurately and rapidly distinguished, the variety of the Paeonia sect. Moutan to be detected is determined, efficient, low-cost and convenient-to-operate Paeonia sect. Moutan variety identification is realized, and the method can beused for molecular identity card construction, variety identification, and genetic diversity analysis of the Paeonia sect. Moutan variety and is very wide in application prospect.

Description

technical field [0001] The invention relates to the technical field of plant molecular marker assisted breeding, in particular to an SSR molecular marker for identifying varieties of purple spot peony and its application. Background technique [0002] Peony (Paeonia sect. Moutan) is a big and colorful flower, loved by people all over the world, and is a traditional famous flower in China. At present, there are more than 2,000 species of peony in the world, and about 17 species groups (Cheng, 2007). As the origin of peony, China has a history of more than 1,600 years of cultivation and breeding, and has accumulated a large number of excellent cultivars. There are more than 1,000 varieties, mainly including four major cultivar groups: Central Plains, Northwest, Jiangnan, and Southwest. The variety group of Paeonia rockii is also called the Northwest peony variety group. It is famous for having an obvious purple spot at the base of the petals and has important ornamental value...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
Inventor 成仿云郭鑫
Owner BEIJING FORESTRY UNIVERSITY
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