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Rapid tissue culture method of passion fruits

A passion fruit, fast technology, applied in the field of rapid tissue culture of passion fruit, can solve the problems of short seedling growth cycle, bud proliferation coefficient, etc., and achieve the effect of good water permeability, rich nutrition, and well-developed root system

Pending Publication Date: 2019-06-14
广西福桐林业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the problems existing in the existing passion fruit propagation, and provides a rapid tissue culture method for passion fruit with short seedling cultivation period, high survival rate, high bud multiplication coefficient, easy rooting seedlings and robustness, and easy seedling hardening

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of passion fruit rapid tissue culture method, concrete operation steps are as follows:

[0029] (1) Acquisition of explants and pretreatment: Select robust 2-year-old passion fruit plants of the variety "Tainong" without pests and diseases, cut off 3-5cm stem segments, wash them with warm water for 5 times, and put them in alcohol and Soak in the disinfectant solution of oligochitosan for 30min, the concentration of described disinfectant alcohol is 10%, the consumption of oligochitosan is 5g / L, clean up with clear water again, the moisture on explant surface is blotted dry with sterilized paper;

[0030] (2) First-generation induction: Cut the sterilized passion fruit explants horizontally every 0.2cm-0.4cm, and use the blade to make several cuts longitudinally on the surface of the stem section, and then inoculate it on the medium for inducing callus On the above, the callus was obtained by culturing at a temperature of 22±2°C, in dark culture, and in a sterile...

Embodiment 2

[0037] A kind of passion fruit rapid tissue culture method, concrete operation steps are as follows:

[0038] (1) Acquisition of explants and pretreatment: Select robust 1-year-old passion fruit plants of the variety "Gypsophila" that are free from diseases and insect pests, cut off 3-5cm stem segments, wash them with warm water for 3 times, and put them in alcohol containing Soak in the disinfectant solution of oligochitosan for 20min, the alcohol concentration of said disinfectant is 5%, the dosage of oligochitosan is 8g / L, then clean it with clear water, and dry the moisture on the explant surface with sterilized paper ;

[0039] (2) First-generation induction: Cut the sterilized passion fruit explants horizontally every 0.2cm-0.4cm, and use the blade to make several cuts longitudinally on the surface of the stem section, and then inoculate it on the medium for inducing callus On the above, the callus was obtained by culturing at a temperature of 22±2°C, in dark culture, and...

Embodiment 3

[0046] A kind of passion fruit rapid tissue culture method, concrete operation steps are as follows:

[0047] (1) Acquisition of explants and pretreatment: Select robust 2-year-old passion fruit plants with the variety "Golden" that are free from diseases and insect pests, cut off 3-5cm stem segments, wash them with warm water for 3 times, and put them in alcohol and husks Soak in the disinfectant solution of oligosaccharides for 25min, the alcohol concentration of the disinfectant solution is 7.5%, the dosage of chitosan oligosaccharide is 10g / L, then clean it with clear water, and dry the moisture on the explant surface with sterile paper;

[0048] (2) First-generation induction: Cut the sterilized passion fruit explants horizontally every 0.2cm-0.4cm, and use the blade to make several cuts longitudinally on the surface of the stem section, and then inoculate it on the medium for inducing callus On the above, the callus was obtained by culturing at a temperature of 22±2°C, i...

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Abstract

The invention relates to a rapid tissue culture method of passion fruits. The method comprises the steps of explant acquisition and pretreatment, primary induction, subculture, rooting culture, bud strengthening culture and acclimatization and transplant. The rapid tissue culture method of the passion fruits has the advantages that various factors and culture methods such as the explant acquisition and pretreatment, primary induction, subculture, rooting culture and bud strengthening culture of the passion fruits are screened, and appropriate seedling hardening and raising measures are adopted, so that a passion fruit tissue culture rapid propagation technical system is established, the system has the advantages that the seedling raising cycle is short, the survival rate is high, the bud multiplication coefficient is high, seedlings easy to root are healthy and strong, and no virus exists, technical support is provided for passion fruit tissue culture industrialized seedling production, and the method is of great significance to promote the development of the passion fruit industry.

Description

technical field [0001] The invention relates to the technical field of passion fruit propagation, in particular to a rapid tissue culture method for passion fruit. Background technique [0002] Passion fruit (Passiflora edulia Sims), also known as Passiflora, is a herbaceous vine of Passiflora genus Passiflora, native to Brazil in South America, and is now widely planted in tropical and subtropical regions. It is planted in Fujian, Guangdong, Hainan, Guangxi, Yunnan, Sichuan and other provinces. Passion fruit juice has good color, fragrance and taste, and is rich in nutrition. It contains 17 essential amino acids, multivitamins, trace elements and other beneficial ingredients. The majority of consumers love. With the deepening of people's understanding of the nutritional value of passion fruit, the consumption of passion fruit has increased year by year, and the planting area has continued to expand, making seedling propagation unable to meet the needs of production develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 郑兴华
Owner 广西福桐林业科技有限公司
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