Method of preparing citrus pectin oligosaccharide under weak electrolysis condition

Abstract

The invention relates to a method of preparing citrus pectin oligosaccharide under a weak electrolysis condition. The method comprises following steps: dissolving high ester citrus pectin in a sodiumchloride solution to prepare a pectin solution; adding the pectin solution into an electrolytic tank (A) taking graphite as the negative pole; adding a sodium chloride solution with an equal concentration into an electrolytic tank (B) taking a platinum electrode as the positive pole; carrying out DC electrolysis under a weak electrolysis condition (voltage is 15 to 25V), stirring the solutions during the electrolysis process; collecting obtained electrolytic solution in the electrolytic tank (A), adjusting the pH to 6-7 by using hydrochloric acid; adding anhydrous ethanol to carry out precipitation, wherein the ratio of anhydrous ethanol to the electrolytic solution is 4:1; collecting the precipitates, and drying to obtain pectin oligosaccharide. By adopting the provided method, macromolecular high ester citrus pectin is electrolyzed to obtain micromolecular pectin oligosaccharide; by controlling the electrolysis voltage and time, the degradation degree can be controlled so as to obtain pectin oligosaccharide (POS) with a molecular weight of 3000 to 8000, and the commodity value is increased. Moreover, the method is environmentally friendly and can be applied to industrial production easily, and the power consumption is low.

Description

technical field [0001] The invention relates to a method for preparing citrus pectin oligosaccharides, in particular to a method for preparing citrus pectin oligosaccharides under weak electrolysis conditions. Background technique [0002] Oligosaccharides are the most common dietary prebiotics, which can be fermented and utilized by gastrointestinal flora to change the composition and (or) activity of microorganisms, thereby promoting the health of the host body. Pectin oligosaccharides (pectic oligosaccharides, POS) are low molecular weight oligosaccharide products formed after depolymerization of pectin, and are a new type of prebiotics. POS is usually prepared from agricultural waste through physical, chemical and biological enzyme methods. Due to the complex composition of raw materials, the yield and purity of POS are low, and further separation and purification of POS are required, which limits the application of POS to a certain extent. scope. At present, products ...

AI Technical Summary

Problems solved by technology

POS is usually prepared from agricultural waste through physical, chemical and biological enzyme methods. Due to the complex composition of raw materials, the yield and purity of POS are low, and further separation and purification of POS are required, which limits the application of POS to a certain extent. scope

At present, products containing pectin oligosaccharides are scarce and expensive in the market; the main constraint is that the POS preparation technology is immature, and large-scale production cannot be formed

The reported POS preparation methods (physical methods, chemical methods, and biological enzyme methods, etc.) have problems such as excessive pectin degradation or incomplete pectin degradation, complex product components, high production costs, high energy consumption, and environmental pollution. Scale Preparation Requirements